OBJECTIVE To describe isolation and in vitro maturation of primary oocytes from the ovarian cortex in the presence of hypothalamic pituitary down-regulation. DESIGN Case report. SETTING Tertiary care university-affiliated hospital. PATIENT(S) An 18-year-old patient was given treatment with the ABVD (Adriamycin, bleomycin, vinblastine, and dacarbazine) protocol for Hodgkin's disease. She u...

Children that undergo treatment for cancer are at risk of suffering from subfertility or hormonal dysfunction due to the detrimental effects of radiotherapy and chemotherapeutic agents on the gonads. Cryopreservation of ovarian tissue prior to treatment offers the possibility of restoring gonadal function after resumption of therapy. Effective counseling and management of pediatric patients is ...

Background Fertility preservation for female cancer patients prior to cancer therapy has emerged as an essential component of comprehensive patient care. In the US, approximately 6% of women diagnosed with invasive cancer between 2007 and 2011 that were under 45 years old. The incidence of cancers in reproductive age women signifies a need for fertility preservation options and this need is inc...

Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovin...

Using patient-derived xenografts (PDXs) for preclinical cancer research demands proper storage of tumour material to facilitate logistics and to reduce the number of animals needed. We successfully established 45 subcutaneous ovarian cancer PDXs, reflecting all histological subtypes, with an overall take rate of 68%. Corresponding cells from mouse replaced human tumour stromal and endothelial c...

The cryopreservation of ovarian tissues is a technology with significant potential for the preservation of the genetic resource materials of working dogs, including guide dogs for the blind. However, no attempt has been reported on cryopreservation of the canine ovary. Thus, we evaluated a vitrification method for cryopreservation of canine ovaries and determined the potential functionality of ...

AIM To investigate possible associations between serum anti-Müllerian hormone (AMH) levels, the number of oocytes collected and the availability and suitability of produced embryos for cryopreservation in in-vitro fertilization (IVF) cycles. PATIENTS AND METHODS Sixty women in their first IVF/intracytoplasmic sperm injection (ICSI) cycle were studied. The short stimulation protocol was used f...

The risk of developing premature ovarian failure and azoospermia is a major concern in long-term survivors treated for Hodgkin's lymphoma. Alkylating chemotherapy containing procarbazine and/or cyclophosphamide causes prolonged azoospermia in 90-100% of men and premature ovarian failure in 5-25% of women under the age of 30. The risk of infertility increases with the cumulative dose of alkylati...

Cryopreservation of ovarian cortex has important implications in the preservation of fertility and biodiversity in animal species. Slow freezing of cat ovarian tissue resulted in the preservation of follicular morphology and in the follicular development after xenografting. Vitrification has been recently applied to ovarian tissues of different species, but no information is available on the ef...

OBJECTIVE To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. DESIGN Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. SETTING University-affiliated laboratory. ANIMALS Rhesus monkey ovarian tissue. INTERVENTION(S) No...