Salmonella phage P22 has been used in the construction of three sorts of specialized transducing phage: P22 proAB, P22 proABlac and P22 argF. The bacterial genes carried are derived from E. coli K12. Since E. coli and Salmonella chromosomes recombine very poorly, E. coli genes cannot be transduced into Salmonella recipients by P22‘s generalized transduction mechanism. Therefore, stable inherita...

Methods 1) P22: The P22 protein cage (60 nm) is bioengineered with an internal polymer network with amine functional groups allowing incorporation of ~9100 Gd-DTPA molecules per cage via the amine groups (Figure 1: [1]). This provides a per cage relaxivity of 70000 mMs, superior to Gd-DTPA for the equivalent Gd concentration. 2) Atherosclerosis Models: Both ApoE-deficient (ApoE) and FVB mice we...

Among the components of the RNA silencing pathway in plants, RNA-dependent RNA polymerases (RDRs) play fundamental roles in antiviral defence. Here, we demonstrate that the Nicotiana benthamiana RDR6 is involved in defence against the bipartite crinivirus (genus Crinivirus, family Closteroviridae) Tomato chlorosis virus (ToCV). Additionally, by producing a p22-deficient ToCV infectious mutant c...

In the absence of host RecA function, plasmid transduction by bacteriophage P22 can be mediated by Erf recombinase. Erf is not carried on the infecting particle but synthesized upon infection. In the recipient cell, Erf can promote both generalized plasmid transduction (which requires the circularization of plasmids transduced as linear multimers) and specialized plasmid transduction (which req...

Bacteriophage P22 has long been considered a hallmark model for virus assembly and maturation. Repurposing of P22 and other similar virus structures for nanotechnology and nanomedicine has reinvigorated the need to further understand the protein-protein interactions that allow for the assembly, as well as the conformational shifts required for maturation. In this work, gp5, the major coat struc...

(Received 10 NovembPr IYSO, and in revised form 24 April 19X1) p12 cly mutants lysogenize at very high frequency after infection of wild-type Salmonella. One class of P22 cly mutations, clyA mutations, are shown to map within the P22 cl gene and to increase the activity of t,he cl gene product during establishment of lysogeny. The P22 clyil mutations also appear to affect c%-repressor gene expr...

The dissimilar combination of 2.25Cr-1Mo (P22) and modified 9Cr-1Mo (P91) obtained using Gas Tungsten Arc Welding (GTAW) process employing the Ni-based superalloy filler ERNiCr-3 (IN82) ERNiCrMo-3 (IN625) have been investigated for microstructure evolution mechanical properties. butt weld joint was produced single bevel groove geometry. structural integrity welded measured in respect tensile st...

Although large numbers of temperate phages are known to exist in nature, only a small number of these have been examined in much detail. Foremost among these is the coliphage ~, which has been studied in great depth over the past two decades. However, it is already quite clear that phage ~ represents only one alternative among many with respect to any of its interesting properties. Most other t...

Monitoring the complex transmission dynamics of a bacterial virus (temperate phage P22) throughout a population of its host (Salmonella Typhimurium) at single cell resolution revealed the unexpected existence of a transiently immune subpopulation of host cells that emerged from peculiarities preceding the process of lysogenization. More specifically, an infection event ultimately leading to a l...