The measurement of folate in red blood cells (RBCs) is preferred since it reflects long-term folate status in the body compared to plasma/serum folate which may be influenced by recent dietary intake. The commonly accepted technique for RBC folate analysis involves preparation of a hemolysate using a fresh whole blood sample. Hematocrit and plasma folate concentrations are needed to calculate R...

HYPOTHESIS Perioperative events of patients undergoing living donor (LD) right hepatectomy are similar to those of patients undergoing right hepatectomy for disease (DZ). DESIGN Institutional review board-approved retrospective case-control study. SETTING Eight hundred-bed tertiary care referral center. PATIENTS AND METHODS We matched 40 patients who had LD with 40 patients who had DZ. Pe...

Various counting methods have been described and reported for process control of leuco-epleted blood components. The recent production of high-efficiency leucocyte removal filters intensifies the need for sensitivity in determining the ever lower residual concentration of white cells (WBCs) in filtered units. In order to assess which method was the most efficient and feasible in the laboratory ...

BACKGROUND Little is known about the effect of transfusing fresh frozen plasma on the outcome after hepatectomy for hepatocellular carcinoma. PATIENTS AND METHODS Among 410 patients who underwent curative resection between 1992 and 2005, 180 patients had perioperative transfusion with whole blood or packed red blood cells and fresh frozen plasma (group A), while 46 patients were only transfus...

Red cell enzyme activity was m easured in packed red cells obtained from spun hematocrit tubes and compared with results obtained using standard methods (washed, filtered, red blood cells [WFRBC]) in order to evaluate the feasibility of using heel puncture as a means of obtaining accurate quantitative red cell enzym e data in the new born period. Glucose-6phosphate dehydrogenase (G-6-PD) and py...

OBJECTIVE The hemoglobin (Hb) content of packed red blood cells (pRBCs) differs in standard volume units. The pRBC transfusions are based on the number of units routinely. We aimed to use pRBCs according to total Hb content and compare the rates of achieving the target Hb concentration levels with the current transfusion practice. MATERIALS AND METHODS Eighty-nine patients (55 males and 34 fe...

Blood transfusion is an essential component in modern healthcare. being irreplaceable resource needs to be appropriately utilized with preferably minimal or zero percent wastage. Only one third of required blood units are collected a Northern Tanzania Hospital, demanding the need for rate discard and products. This study aimed determine reasons discarding products at Zone Transfusion Centre Tan...

A disposable on-chip whole blood/plasma separator, which is able to separate plasma from whole blood by capillary movement through a bead-packed microchannel, has been designed, fabricated and characterized in this work. Various sized silica beads were packed inside a microchannel using a slurry-bump pattern that prevented the beads from passing through the microchannel. The bead-packed microch...

Selenium (Se) levels in whole blood, red blood cells, and plasma taken from 199 healthy subjects were investigated and found to be 101.1 micrograms/1 (whole blood), 133.1 micrograms/1 (packed red blood cells), and 78.0 micrograms/1 (plasma). In 62 samples of urine, the selenium level, expressed as micrograms/g creatinine, was 11.4. The mean whole blood selenium concentration was significantly h...

Because raised plasma concentrations of atrial natriuretic peptide indicate volume expansion, we studied the effect of red cell transfusion on plasma atrial natriuretic peptide concentration, packed cell volume, and intravascular volume in eight preterm infants. Red cell transfusion increased red cell mass, packed cell volume and erythrocyte count, but decreased plasma volume. Total blood volum...