Paenibacillus larvae is a Gram positive bacterium and the causative agent of the most widespread fatal brood disease of honey bees, American foulbrood (AFB). A total of thirty-three independent Paenibacillus larvae isolates from various geographical origins in North America and five reference strains were investigated for genetic diversity using multilocus sequence typing (MLST). This technique...

American foulbrood is a disease of larval honeybees (Apis mellifera) caused by the bacterium Paenibacillus larvae. Over the years attempts have been made to develop a selective medium for the detection of P. larvae spores from honey samples. The most successful of these is a semiselective medium containing nalidixic acid and pipermedic acid. Although this medium allows the growth of P. larvae a...

Paenibacillus larvae (P. larvae) is a pathogenic bacterium causing American Foulbrood Disease (AFB) in honeybee larvae. It necessary to develop alternative strategies for the control of this disease due serious colonies loses and use antibiotics. Recent studies are aimed at investigating natural fighting agents against P. In our study, it was demonstrate potential antibacterial efficacy ethanol...

In the summer of 1997, larvae of the oriental beetle, Blitopertha orientalis, with milky disease were collected from a turf field in Chiba Prefecture, Japan. Characteristic footprint-shaped sporangia containing a spore and a parasporal body were isolated from the larval hemolymph. Examination of the bacteriological characteristics of this strain revealed that it was unable to grow in 2% NaCl, w...

INTRODUCTION Paenibacillus larvae larvae (WHITE) is the causative agent of American foulbrood (AFB) in honey bees (Apis mellifera L.). This disease is lethal to honey bee colonies if curative control actions are not taken (HANSEN and BRØDSGAARD, 1997). Recently, increasing problems with AFB in Europe (DROBNÍKOVÁ,1992; HANSEN,1996; OTTEN, 1991; VON DER OHE et al. 1996) have renewed the interest ...

American foulbrood, caused by Paenibacillus larvae subsp. larvae (White 1906) is one of the most serious diseases of honey bees, causing beekeepers and health workers to make difficult, complex decisions and leading to the development of 'organic' treatments, such as grapefruit seed extract, with minor residue problems in the end product. This study evaluates the chemical composition of grapefr...

Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honey bee health worldwide. The present study investigates the effect of bodily fluids from honey bee larvae on growth velocity and transcription for this Gram-positive, endospore-forming bacterium. It was observed that larval fluids accelerate the growth and lead to higher bacterial densities during stationary ...