The p21-activated kinase (PAK) family plays a versatile role in cell signaling by forming a hub of interactions. PAKs bind the GTPases like RAC and CDC42. Their proline-rich motifs bind SH3 adaptor proteins such as PIX and NCK. PAKs display nuclear localization signal sites and a potential Integrin binding site. No fully complete structure of the PAKs has been published; partial 3D structures o...

The Shank/ProSAP family of multidomain proteins is known to play an important role in organizing synaptic multiprotein complexes. Here we report a novel interaction between Shank and beta PIX, a guanine nucleotide exchange factor for the Rac1 and Cdc42 small GTPases. This interaction is mediated by the PDZ domain of Shank and the C-terminal leucine zipper domain and the PDZ domain-binding motif...

Previously, we showed PAK-PIX-GIT targets and regulates focal adhesions; here, we uncover a different function for the complex at the centrosome. Active PAK1 is particularly evident in mitosis and phosphorylates the centrosomal adaptor GIT1 on serine 517. Interestingly, direct centrosome targeting activates the kinase via a process not requiring Rho GTPases; excision of the centrosome prevents ...

Sarcasm Language Communication in Seloka Pandir and Pak Kaduk ABSTRACT The creative work of traditional Malay poetry is recording the story a life filled with examples. Traditional serves as main communication tool used by ancestors until now. Among poems involved are seloka; its original function was created to provide entertainment society raising certain icons references. This study focuses ...

BIOCHEMICAL AND STRUCTURAL CHARACTERIZATION OF THE PAK1LC8 INTERACTION. Christine M. Lightcap Shangjin Sun, James D. Lear, Ulrich Rodeck, Tatyana Polenova, and John C. Williams Department of Biochemistry and Molecular Biology & Kimmel Cancer Center, Department of Dermatology, Thomas Jefferson University; Department of Chemistry and Biochemistry, University of Delaware; Department of Biochemistr...

When designing password-authenticated key exchange protocols (as opposed to key exchange protocols authenticated using cryptographically secure keys), one must not allow any information to be leaked that would allow veriication of the password (a weak shared key), since an attacker who obtains this information may be able to run an oo-line dictionary attack to determine the correct password. We...

When designing password-authenticated key exchange protocols (as opposed to key exchange protocols authenticated using cryptographically secure keys), one must not allow any information to be leaked that would allow verification of the password (a weak shared key), since an attacker who obtains this information may be able to run an off-line dictionary attack to determine the correct password. ...

In 2011, the United States Department of Agriculture conducted a project in which elk (Cervus elaphus spp.), white-tailed deer (WTD) (Odocoileus virginianus), and reindeer (Rangifer tarandus) were evaluated by the single cervical tuberculin test (SCT), comparative cervical tuberculin test (CCT), and serologic tests. The rapid antibody detection tests evaluated were the CervidTB Stat-Pak (Stat-P...

Neurofibromatosis type 2 (NF2) is an autosomal dominant disorder characterized by the development of multiple tumors in the central nervous system, most notably schwannomas and meningiomas. Mutational inactivation of NF2 is found in 40-60% of sporadic meningiomas, but the molecular mechanisms underlying malignant changes of meningioma cells remain unclear. Because group I p21-activated kinases ...

Endothelial cells lining the vasculature have close cell-cell associations that maintain separation of the blood fluid compartment from surrounding tissues. Permeability is regulated by a variety of growth factors and cytokines and plays a role in numerous physiological and pathological processes. We examined a potential role for the p21-activated kinase (PAK) in the regulation of vascular perm...