ers of <20 μL often leak while 30 μL of wax results in approximately a 2 mm space between the upper and lower aqueous reagents. Second, the temperature at which mixing occurs depends on both the melting point of the wax and the amount of wax present. We have found that the upper aqueous layer does not enter the bottom layer until the tube has reached >80°C. We determined when the upper aqueous ...

hazelnuts are usually propagated by suckers or layering. however, other methods of propagation have been tested with variable results. grafting is a method that has a few advantages such as fast multiplication rate, earlier fruiting and reducing sucker removal cost on specific rootstocks. unfortunately, grafting is difficult due to slow formation of callus in hazelnuts. here, we report the hypo...

Aim-To identify Candida species in formalin fixed, paraffin wax embedded tissue by sequencing candidal rDNA.Methods-Target rDNA sequences were amplified by polymerase chain reaction (PCR) from fresh isolates of Candida and from 18 preserved oral mucosal tissue samples (16 cases of chronic hyperplastic candidiasis and two fibroepithelial polyps), shown histologically to contain Candida. Identifi...

The use of interphase fluorescence in situ hybridisation (FISH) to study cytogenetic abnormalities in routinely fixed paraffin wax embedded tissue has become commonplace over the past decade. However, very few studies have applied FISH to routinely fixed bone marrow trephines (BMTs). This may be because of the acid based decalcification methods that are commonly used during the processing of BM...

Rabbit polyclonal antibodies were raised against a proline rich, peptide sequence, comprising 13 amino acids, in the cytoplasmic domain of the CD3 epsilon chain. Immunoprecipitation experiments showed that this antibody preparation recognised the CD3 antigen on human T lymphoblasts. The antibody stained normal T cells strongly in tissue sections which had been fixed in formalin or Bouin's solut...

This paper presents a novel latchable phase-change microvalve that can potentially be used for flow gating in portable lab-on-a-chip systems here minimal energy consumption is desired. The microvalve innovatively exploits paraffin wax of low melting point, whose solid–liquid phase hanges allow the closing and opening of fluid flow through deformable microchannel ceiling. The latchable phase-cha...

To investigate the distribution of tumour cells expressing the SYT-SSX fusion gene in biphasic synovial sarcoma, modified reverse transcription polymerase chain reaction (RT-PCR) analysis was performed using microdissected specimens from haematoxylin and eosin stained sections of archival paraffin wax embedded tissues. This modified RT-PCR included a stage with degenerate oligonucleotide primed...