In Saccharomyces cerevisiae, DNA double-strand breaks (DSBs) initiate meiotic recombination at open sites in chromatin, which display a meiosis-specific increase in micrococcal nuclease (MNase) sensitivity. The arg4 promoter contains such a DSB site. When arg4 sequences are placed in a pBR322-derived insert at HIS4 (his4 :: arg4 ), the presence of strong DSB sites in pBR322 sequences leads to a...

Pyrolysis mass spectrometry (Py-MS) has been used to discriminate between four very closely related strains of Escherichia coli; a parent strain UB5021 and three derivatives each containing one of the antibiotic resistance plasmids, pBR322, pACYC184 or R388.

The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general for elements that increase resistance to tetracycline.

Source and Description of Clone: pBT A528 contains 920 bp of the inhibin {3B genomic sequence inserted into pBR322 (1). Polymorphism: BamHI identifies two alleles: Allele AI-4.4 kb, Allele A2-3.4 kb.

Maintenance of plasmids pBR322 and pUC8 in Escherichia coli that was nonculturable after exposure to seawater was studied. E. coli JM83 and JM101, which contained plasmids pBR322 and pUC8, respectively, were placed in sterile artificial seawater for 21 days. Culturability was determined by plating on both nonselective and selective agar, and plasmid maintenance was monitored by direct isolation...

pBR322 DNA/Restriction endonuclease/8-methoxypsoralen/Black light/Crosslink The pBR322 DNA was irradiated with black light in the presence of 8-methoxypsoralen and digested by various restriction endonucleases including Hin dIII and Rsa I. It was found that for every restriction enzymes examined digestion products specific for pretreated DNA appeared as expected from the inhibition of cleavage ...

The fluorescence of ethidium bromide (EB) bound to equimolar amounts of supercoiled form I and unstrained linear form III pBR322, SV40 and PM2 DNA in agarose gels has been measured by scanning a photographic negative of the gel with a microdensitometer. For SV40 and PM2 DNA, commonly used staining conditions cause both forms, i.e. linear and supercoiled, to fluoresce to the same extent. This ob...

The construction of plasmids containing T7 class I promoters with deletion mutants was described. Restriction fragments, ending at the Hinf I site located at position -10 in the promoter from 14.8% of the T7 genome, were cloned into pBR322. This produced the deletion of either the left or the right part of the promoter. The in vitro transcription properties of these plasmids were determined. Co...

Sau 3A1 partial digestion fragments from Bacillus thuringiensis var. kurstaki HD-1 plasmid DNA were ligated into the BamHI site of the cloning vector pBR322 and transformed into Escherichia coli strain HB101. Colonies presumed to contain recombinant plasmids were screened for production of an antigen that would react with antibody made against B. thuringiensis crystals. One strain, ES12, was is...