Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by ...

BACKGROUND & OBJECTIVES The conventional techniques used in TB diagnosis like AFB (acid fast bacilli) smear microscopy lack sensitivity and the gold standard, culture test takes time. A test based on multiplex polymerase chain reaction (PCR) targeting the 38 kDa gene and IS6110 insertion sequence, specific to Mycobacterium tuberculosis was developed to further increase the sensitivity of a TB-P...

BACKGROUND Recently, BRAF inhibitors showed dramatic treatment outcomes in BRAF V600 mutant melanoma. Therefore, the accuracy of BRAF mutation test is critical. METHODS BRAF mutations were tested by dual-priming oligonucleotide-polymerase chain reaction (DPO-PCR), direct sequencing and subsequently retested with a real-time PCR assay, cobas 4800 V600 mutation test. In total, 64 tumors includi...

this study was performed to detect cultivable canine gastric helicobacter-like organisms (ghlo’s) and to evaluate their sensitivity to common antibiotics in two groups of naturally infected dogs (pets and stray dogs). gastric samples were taken from the body and antrum of 30 pets and 30 stray dogs. from each part of canine gastric mucosa, four gastroscopic samples were used for impression smear...

background and aims: influenza a virus infects birds and some mammalian including human. h1 and h3 subtypes are circulated in both human and birds’ population. to determine the prevalence of the mentioned subtypes in birds, different captive bird species in tehran zoo, and affiliated centers in tehran were investigated for virus infection. methods: in this study, between november 2008 and febru...

Objective: The mean platelet volume lymphocyte ratio (MPVLR) and (MPVPR) reflect inflammation more effectively strongly than MPV, lymphocyte, platelet, neutrophil counts separately, we aimed to evaluate the importance of MPVLR MPVPR in COVID-19 patients. Material Methods: Patients with were divided into two groups based on whether their RT-PCR test resulted a positive or negative result. MPVLR,...

Objective: To determine the diagnostic value of rapid COVID-19 antigen test (Panbio, Abbott) in comparison with Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) suspected community cases SARS-CoV-2 infection at a tertiary care center Pakistan 
 Study Design: Cross sectional study.
 Place and Duration Study: Pathology Department, Combined Military Hospital, Lahore Pakistan, fro...

Tuberculosis has remained to be a major public health problem in Nepal. The risk of spread of infection and emergence of drug-resistant strain has created the need for a rapid, sensitive and specific diagnostic test. In addition, clinically suspicious cases that do not give positive result in conventional laboratory test need more sensitive test for diagnosis. In order to evaluate the possibili...

INTRODUCTION A semi-automated magnetic capture probe-based DNA extraction and real-time PCR method (MC-PCR), allowing for a more efficient large-scale surveillance of Echinococcus multilocularis occurrence, has been developed. The test sensitivity has previously been evaluated using the sedimentation and counting technique (SCT) as a gold standard. However, as the sensitivity of the SCT is not ...