Autophagy Deficiency Promotes -Lactam Production in Penicillium chrysogenum † Magdalena Bartoszewska, Jan A. K. W. Kiel, Roel A. L. Bovenberg, Marten Veenhuis, and Ida J. van der Klei* Molecular Cell Biology, and Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, P.O. Box 14, 9750 AA Haren, Netherlands, and DSM Biotechnology Center, P.O. Box 425, 2600 AK, Delft, Ne...

The gene encoding an aromatic and neutral aliphatic amino acid permease of Penicillium chrysogenum was cloned, functionally expressed and characterized in Saccharomyces cerevisiae M4276. The permease, designated PcMtr, is structurally and functionally homologous to Mtr of Neurospora crassa, and unrelated to the Amino Acid Permease (AAP) family which includes most amino acid permeases in fungi. ...

The presence of a tricarboxylic acid cycle in Penicillium chry8ogenum, as yet, has not been demonstrated conclusively. Ajl (1951) stated that of the mechanisms proposed for oxidation of the 2 carbon fragment in mold metabolism, the evidence for a tricarboxylic acid cycle is the least convincing. This paper presents a study of some of the enzymes of the tricarboxylic acid cycle in cell-free prep...

The naphthoquinones lapachol and dichloroallyl lawsone readily undergo oxidative ring fission when incubated with several fungi and streptomycetes. Penicillium notatum was employed to produce the ring fission product of dichloroallyl lawsone which was isolated and characterized by spectral analyses and chemical synthesis. The mechanism of oxidative ring fission of lapachol was studied by growin...

In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products belong to the SulP superfamily of sulfate permeases were isolated. Transformation of a sulfate uptake-negative sB3 mutant of Aspergillus nidulans with the sutB gene completely restored sulfate upta...

Marine environments are unique habitats for microorganisms and represent uninvestigated areas that possess valuable resources. Fungi may also be important because they contribute to marine ecosystems as decomposers. The aims of this study were to investigate the halotolerance of marine-derived fungi and their enzymatic properties. Eighteen fungal strains representing 11 different species were u...

Endophytic fungus Penicillium chrysogenum, a new source of hypocrellins Liyuan Meng , Peng Sun , Hua Tang , Ling Li , Siegfried Draeger , Barbara Schulz , Karsten Krohn , Hidayat Hussain , Wen Zhang *, Yanghua Yi a,** Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, 325 Guo-He Road, Shanghai 200433, PR China b Institut für Mikrobiologie, Technische Unive...

The relation of CO2 to the metabolism of Penicillium chrysogenum has been studied in three ways in this work: (1) attempts were made to replace C02; (2) radioactive C1402 was used to determine the conditions under which CO2 fixation occurred; and (3) attempts were made to identify some of the products of CO2 fixation, especially amino acids. These methods, singly or in combination, have been us...

Species classified in Penicillium sect. Chrysogena are primary soil-borne and the most well-known members are P. chrysogenum and P. nalgiovense. Penicillium chrysogenum has received much attention because of its role in the production on penicillin and as a contaminant of indoor environments and various food and feedstuffs. Another biotechnologically important species is P. nalgiovense, which i...

The stability of the antifungal activity of flaxseed (Linum usitatissimum) protein extract was evaluated in this study. Response surface methodology (RSM) using Box-Behnken factorial design was used to evaluate the effects of treatment variables, that is, temperature (50 to 90 degrees C), time (1 to 29 min), and pH (2 to 8), on the residual antifungal activity (RAA) against Penicillium chrysoge...