SARS-CoV-2, a single stranded positive RNA-virus, emerged in 2019 and caused global pandemic. Some infected patients develop severe complications, such as acute respiratory distress syndrome (ARDS) pulmonary fibrosis. The mechanisms leading to lung fibrosis the context of COVID-19 are still unclear. However, there correlations between this condition certain inflammatory mediators that elevated ...

BACKGROUND Recent research has demonstrated that circulating peripheral blood mononuclear fractions (PBMC) containing haematopoietic stem (HSC)/progenitor cells have the potential to play a crucial role in regenerative medicine strategies. Work in our laboratory has shown that a peripheral blood mononuclear cell fraction (PBMC) enhances cartilage repair in an osteochondral defect model in sheep...

Bacterial toxins can exhibit anticancer activities. Here we investigated the anticancer effects of the listeriolysin O toxin produced by Listeria monocytogenes. We found that supernatants of Listeria monocytogenes strains (wild type, 1189, and 1190) were cytotoxic to the Jurkat cell line and human peripheral blood mononuclear cells (PBMC) in a concentration-dependent manner. The supernatant of ...

Normal human peripheral blood mononuclear cells (PBMC) and leukaemic cell lines (three of human and one of gibbon origin) were found to contain similar levels of calmodulin (CaM) when expressed relative to the total cell protein. Two of the cell lines examined further were found to contain much higher amounts of CaM per cell (up to 5-fold) than PBMC but this was readily explained by their much ...

background: the ectopic expression of receptor tyrosine kinase ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. here we report, for the first time, expression of ror1 gene in both tumor tissues and peripheral blood mononuclear cells (pbmc) from patients with renal cancer (rc). methods: in the current study,...

Abstract Identifying tumor-specific T cells is critical to the development of T-cell based immunotherapies. As a result, computational tools have been developed predict neoepitopes present within tumor. A common experimental approach used screen neoepitope candidates long-term in vitroexpansion patient peripheral blood mononuclear (PBMC) with candidate peptides-followed by fluorospot detect ant...

Objective: There are a number of medicinal plant products which has been used to treat various immunological diseases. Out of these medicinal plants, Calamus rotang has shown several medicinal properties including anti-viral, anti-diabetic, anti-inflammatory effects. Normally, the roots of C. rotang are used in various ailments to cure piles, burning sensation, cough, leprosy and bleeding disor...

This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins wit...

Abstract Background Traditional laboratory model organisms represent a small fraction of the diversity multicellular life, and findings in any given experimental often do not translate to other species. Immunology research non-traditional can be advantageous or even necessary, such as when studying host-pathogen interactions. However, presents multiple challenges, many stemming from an incomple...

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