Abstract The human prolactin antagonist ? 1-11 -G129R-hPRL is a 21.9 kDa recombinant protein with 188 amino acids that downregulates the proliferation of variety cells expressing receptors. Periplasmic expression proteins in E. coli has been considered an option for obtaining soluble and correctly folded protein, as alternative to cytoplasmic production. aim this work was, therefore, synthesize...

The leucine-specific binding protein of Escherichia coli is a periplasmic protein that is synthesized as a precursor and subsequently is processed during its secretion into the periplasmic space. The processing of both the leucine-specific binding protein and a plasmid-coded beta-lactamase is inhibited by phenethyl alcohol and by the proton ionophore, carbonylcyanide m-chlorophenylhydrazone (CC...

A major component of the Escherichia coli response to elevated medium osmolarity is the synthesis of a periplasmic protein with an Mr of 31,000. The protein was absent in mutants with lambda placMu insertions in the proU region, a locus involved in transport of the osmoprotectant glycine betaine. This periplasmic protein has now been purified to homogeneity. Antibody directed against the purifi...

The OMPs (outer membrane proteins) of Gram-negative bacteria have to be translocated through the periplasmic space before reaching their final destination. The aqueous environment of the periplasmic space and high permeability of the outer membrane engender such a translocation process inevitably challenging. In Escherichia coli, although SurA, Skp and DegP have been identified to function in t...

Cefiderocol is a cephalosporin designed to treat multidrug-resistant Gram-negative infections. By forming chelated complex with ferric iron, cefiderocol transported into the periplasmic space via bacterial iron transport systems and primarily binds penicillin-binding protein 3 (PBP3) inhibit peptidoglycan synthesis. This mode of action results in having greater vitro activity against many bacil...

Characterization of the periplasmic region of PomB, a Na + -driven flagellar 1 stator protein in Vibrio alginolyticus 2 3 4 Na Li 1,2 , Seiji Kojima 2* and Michio Homma 2 5 6 1 Division of Microbiology, Graduate School of Life Science, Northwest A&F 7 University, Yanglin, Shaanxi, Yanglin 712100, China; 2 Division of Biological 8 Science, Graduate School of Science, Nagoya University, Furo-cho,...

A new isoenzyme of carbonic anhydrase has been isolated and purified from Chlamydomonas reinhardtii. This carbonic anhydrase is composed of two nonidentical subunits with apparent molecular masses of 39 and 4.5 kDa and is located in the periplasmic space. This is the second periplasmic carbonic anhydrase found in C. reinhardtii. Two genes, CAH1 and CAH2, which code for carbonic anhydrase, have ...