Phenylalanine ammonia-lyase (PAL) and peroxidase (POD) enzyme activities, total phenolics and pyruvic acid (PA) (as pungency indicator) were measured in onion bulb tissues (c.v. Rouge Amposta) during storage at 4 and 20 3C. After 2 wk, PAL activity increased at 4 3C, but decreased at 20 3C, and after 4 wk, PAL activity at 20 3C was higher than activity observed at 4 3C. Peroxidase activity was ...

The effects of arsenic (As) were investigated on seed germination, root and shoot length and their biomass and some other factors to elucidate the toxicity of As. The results showed low concentrations of As (0-1 mg/kg) stimulated seed germination and the growth of root and shoot, however, these factors all decreased gradually at high concentrations of As (5-20 mg/kg). The contents of O2*-, MDA,...

H2S is a signaling molecule in plants and animals. Here we investigated the effects of H2S on programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain. NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2 (-)), hydrogen peroxide (H2O2), and ma...

A new method for online detection of peroxidase (POD) using 3D printing, active magnetic mixing, fluidic control, and optical detection was developed and demonstrated in this study. The proposed POD detection system consisted of a 3D printing and active magnetic mixing based fluidic chip for online catalytic reaction, an optical detector with a fluidic flow cell for quantitative determination o...

Iron deficiency is one of the most important factors affecting growth and yield. Utilizing tissue culture technique to study the response of plant to Fe-stress was suggested as a rapid and simple method. Fe-deficiency was explored by cultivating shoots of Borage plantlets on MS media with 27.8, 13.0, 0.0 mg/L FeSO4. Results showed that, severe Fe-deficiency induced yellowing and typical iron de...

With the aim to clarifying the role of the ABA/H2O2 signaling cascade in the regulating the antioxidant capacity of grafted cucumber plants in response to Ca(NO3)2 stress, we investigated the relationship between ABA-mediated H2O2 production and the activities of antioxidant enzymes in the leaves of pumpkin-grafted cucumber seedlings. The results showed that both ABA and H2O2 were detected in p...

UNLABELLED Structural changes involved in the reactivation of peroxidases (PODs) from broccoli and horseradish (HRP) following heat denaturation were investigated by using circular dichroism and absorption spectroscopy. Cooling heat-treated enzymes resulted in rapid refolding of the secondary structure into an inactive structural species, similar in conformation to the native enzyme. Reassociat...

To understand the adaptability of alfalfa (Medicago sativa L.) to environmental stresses, we analyzed the activity of several antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX), and catalase (CAT), in alfalfa shoots and roots subjected to salt and drought stresses during germination. The germination rate of six alfalfa cultivars was comparati...

WRAB18, an ABA-inducible protein belongs to the third family of late embryogenesis abundant (LEA) proteins which can be induced by different biotic or abiotic stresses. In the present study, WRAB18 was cloned from the Zhengyin 1 cultivar of Triticum aestivum and overexpressed in Escherichia coli to explore its effects on the growth of E. coli under different abiotic stresses. Results suggested ...

The groundnut, Arachis hypogaea seedlings, when grown in pot cultures for 10-25 days at 25, 50, 100 microM CdCl2, showed a marked decline in growth compared to control. Similar trend was observed for nitrate reductase (NR) and nitrite reductase (NiR) activities whereas proline, peroxidase (POD) and catalase (CAT) showed increasing trend when observed on the 10th day of the experiment. Changes h...