Colloidal stability of IgG antibody solutions is important for pharmaceutical and medicinal applications. Solution pH and ionic strength are two key factors that affect the colloidal stability of protein solutions. In this work, we use a method based on the PEG-induced liquid-liquid phase separation to examine the effects of pH and ionic strength on the colloidal stability of IgG solutions. We ...

Protein conformations change among distinct thermodynamic states as solution conditions (temperature, denaturants, pH) are altered or when they are subjected to mechanical forces. A quantitative description of the changes in the relative stabilities of the various thermodynamic states is needed to interpret and predict experimental outcomes. We provide a framework based on the Molecular Transfe...

lysozyme, as a model protein, was precipitated through the formation of protein-zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (plga) microspheres. various parameters, including ph, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. the resulting protein parti...

1. The rate of inactivation of crystalline trypsin solutions and the nature of the products formed during the inactivation at various pH at temperatures below 37 degrees C. have been studied. 2. The inactivation may be reversible or irreversible. Reversible inactivation is accompanied by the formation of reversibly denatured protein. This denatured protein exists in equilibrium with the native ...

in order to investigate the effects of supplementary calcium chloride and potassium nitrate on plant growth of ammi (carum copticum l.) in different salinity levels and nutrient solutions (ns), an experiment based on randomized complete block design (rcbd) with factorial arrangement was conducted under greenhouse condition in the faculty of agriculture, vali-e-asr university of rafsanjan, durin...

introduction : one of the most important problems of synthetic packaging of materials had been the time consuming process of their decomposition. therefore, they have the potential to contaminate the environment. during the recent years, the above-mentioned problem has paved the way for developing biodegradable biopolymers. one group of these polymers is films and edible coatings. soy protein i...

acid-binding capacity (abc) of a feed is the amount of acid required to reduce the ph of feed to a special level such as 3. all poultry feed ingredients used in this study were collected from feed mills in iran. the ph and abc were determined using the standard methods in deionized and magnetized water at room and body temperatures. the ph of most feed ingredients was raised in magnetized water...

MOTIVATION The identification of suitable conditions for crystallization is a rate-limiting step in protein structure determination. The pH of an experiment is an important parameter and has the potential to be used in data-mining studies to help reduce the number of crystallization trials required. However, the pH is usually recorded as that of the buffer solution, which can be highly inaccura...

background: consistency, backing process and content differences could influence cariogenic potential of foods. the aim was to compare plaque ph changes following consumption of two types of bread with different physical characteristics. methods: in this clinical trial, interproximal plaque ph of 10 volunteers with high risk of dental caries (saliva streptococcus mutans > 10 5 , high dental car...