A promising polycyclic aromatic hydrocarbon-oxidizing P450 CYP5136A3 from Phanerochaete chrysosporium was rationally engineered to enhance its catalytic activity. The residues W129 and L324 found to be critical in substrate recognition were transformed by single (L324F) and double (W129L/L324G, W129L/L324F, W129A/L324G, W129F/L324G and W129F/L324F) mutations, and the engineered enzyme forms wer...

Carbohydrate structures are modified and degraded in the biosphere by a myriad of mostly hydrolytic enzymes. Recently, lytic polysaccharide mono-oxygenases (LPMOs) were discovered as a new class of enzymes for cleavage of recalcitrant polysaccharides that instead employ an oxidative mechanism. LPMOs employ copper as the catalytic metal and are dependent on oxygen and reducing agents for activit...

Biodegradation of the Hexahydro-l,3,5-Trinitro-l,3,5-Triazine Ring Cleavage Product 4-Nitro-2,4-Diazabutanal by Phanerochaete chrysosporium Diane Fournier, Annamaria Halasz, Jim Spain, Ronald J. Spanggord, Jeffrey C. Bottaro, and Jalal Hawari* Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H4P 2R2, Canada; U.S. Air Force Research Laboratory, Tyndall Air ...

The biosorption of heavy metals can be an effective process for the removal of such metal ions from aqueous solutions. In this study, the adsorption properties of nonliving biomass of phanerochaete chrysosporium for Pb (II) and Zn (II) were investigated by the use of batch adsorption techniques. The effects of initial metal ion concentration, initial pH, biosorbent concentration, stirring speed...

Various species of Absidia, Aspergillus, Cunninghamella, Trichothecium, Penicillium, and Phanerochaete were found to transform rotenone to one or more metabolites. Two biotransformation products were isolated from a preparative-scale incubation of rotenone with Cunninghamella blakesleeana and identified as 1',2'-dihydro-1',2'-dihydroxyrotenone and 3'-hydroxyrotenone (amorphigenin). The catalyti...

A selective medium was developed that is capable of isolating Phanerochaete chrysosporium from soil. This medium contains 15 ppm of benomyl (15 mug g) and 550 ppm of streptomycin sulfate in 2% malt agar and is held at 39 degrees C after inoculation. P. chrysosporium was isolated from three nonsterile forest soils to which the fungus had been added. These soils contained large microbial populati...