Recombinant proteins are widely used in biomedical sciences, and in pharmaceutical research. Through genetic recombination, specific DNA sequences are engineered and inserted into a biological host. Once inside the host, the DNA is transcribed and translated into the target protein and is ready to be purified. Here, we describe the transfection, purification, and visualization of Fc-tagged SEMA...

Electrospray ionization - ion mobility spectrometry - mass spectrometry (ESI-IMS-MS) allows the identification of protein polymorphic distribution of protein conformers and oligomers. We report the detailed identification of the species observed with commercially available pharmaceutical preparation of wild-type, regular human insulin.

Protein aggregation is a very fascinating matter due to its implication in many human neurodegenerative diseases and its relevance in food and pharmaceutical industries. In some cases, the aggregation of protein is a natural phenomenon occurring in living organisms. For example, in the reaction leading from the globular (G) monomeric actin to its polymeric fibrillar (F) structure (Morris et al....

background: black seed (nigella sativa l.) in traditional medicine is well known that the chemical composition of the immune system with many applications in traditional medicine to fight the infection. this study investigated the effect of the plant's role in the immune system and blood protein. experimental: in this study male mice of balb/c strain were divided into four groups tested. contr...

Related the physio-pathological process of COVID-19 disease it is interesting to focus aspect. Played by interaction Sars-Cov-2 protein with integrins human epithelial pulmonary cell. A bio molecular approach help in deeply verify involved factors and results this Activation RGD mediated. Of Great interest also relationship some vaccine strategy followed various pharmaceutical industry. The wor...

Computer-aided drug design is a signiicant component of the rational approach to pharmaceutical drug design. Chemists now consider the geometric and chemical characteristics of molecules early in the design process in an eeort to quickly identify ligands that have good chances of becoming potent pharmaceutical drugs. Computer assistance is not only helpful but also necessary to narrow down the ...

according to the vital role of pharmaceutical industry in health care system, pharmaceutical supply chain security, standard production and distribution of the pharmaceutical products are of great importance for pharmaceutical companies. therefore, applying technology, especially radio frequency identification (rfid), is essential to achieve these goals. moreover, due to the importance of secur...

hepatitis b virus (hbv) is considered as a global health concern and hepatitis b surface antigen (hbsag) is the most immunogenic protein of hbv. the purpose of this study was to evaluate the expression of hbsag on the cell surface of human embryonic kidney cell line (hek293t). after transformation of expression vector pcdna/hbsag to e.coli top10f’, plasmid was extracted and digested with bglii....