Talaromyces purpurogenus, an endophytic fungus, exhibits beneficial effects on plants during plant–fungus interactions. However, the molecular mechanisms underlying plants’ responses to T. purpurogenus under low-phosphorous (P) stress are not fully understood. In this study, we investigated transcriptomic changes in maize with low-P-sensitive (31778) and -tolerant (CCM454) genotypes low-P its s...

Phosphorylation of secretory and integral membrane proteins and of proteoglycans also occurs in the lumen of the Golgi apparatus. ATP, the phosphate donor in these reactions, must first cross the Golgi membrane before it can serve as substrate. The existence of a specific ATP transporter in the Golgi membrane has been previously demonstrated in vitro using intact Golgi membrane vesicles from ra...

Many bacteria regulate gene expression in response to phosphate availability using a two-component signal transduction system, the activity of which is controlled by interaction with the Pst phosphate specific transporter and a cytoplasmic protein PhoU. Mycobacterium tuberculosis, the causative agent of tuberculosis, requires its phosphate sensing signal transduction system for virulence and an...

We studied the activity of the fluorescently labeled membrane transporter MalGFK(2), which transports maltose at the expense of ATP hydrolysis. We used a commercially available malachite green assay (SensoLyte MG phosphate assay kit; Anaspec) to quantify the liberated phosphate upon ATP hydrolysis. However, strong variations in phosphate concentration were measured when using the supplier's han...

Arbuscular mycorrhizal (AM) fungi live in mutualistic symbioses with plant roots, in which the fungus provides the plant with enhanced mineral uptake from the soil, principally phosphate, in exchange for carbon to support fungal growth (reviewed in Bonfante and Genre, 2010). Phosphate status is a critical parameter controlling the development of the symbiosis, as both high phosphate levels in t...