Reduction of phytate is a major goal of plant breeding programs to improve the nutritional quality of crops. Remarkably, except for the storage organs of crops such as barley, maize and soybean, we know little of the stereoisomeric composition of inositol phosphates in plant tissues. To investigate the metabolic origins of higher inositol phosphates in photosynthetic tissues, we have radiolabel...

The hydrolysis of O-arylphosphorothioates by protein-tyrosine phosphatases (PTPases) was studied with the aim of providing a mechanistic framework for the reactions of this important class of substrate analogues. O-arylphosphorothioates are hydrolyzed 2 to 3 orders of magnitude slower than O-aryl phosphates by PTPases. This is in contrast to the solution reaction where phosphorothioates display...

Enzyme helps fold the SAC E spert et al. identify a phosphatase that helps shut down the spindle assembly checkpoint (SAC) when chromosomes are correctly attached to the spindle. The SAC prevents cells from entering anaphase until they have verifi ed the connections between spindle microtubules and the chromosomes. The checkpoint forms when the enzyme Mps1 phosphorylates the kinetochore protein...

The levels of glucose, sugar phosphates, and adenosine phosphates were determined in primary leaves of intact bean plants during normal senescence and compared to leaves in which senescence was delayed by application of benzyladenine (BA). In both cases there was a rise with time in the levels of glucose 1-phosphate, glucose 6-phosphate, and fructose 6-phosphate, and a decline in 2-phosphoglyce...

Rubinstein J (1995) Cohtmn Flotation: Processes, Designs and Practices. New York: Gordon and Breach. Schubert Hand Bischofberger C (1979) On the optimization of hydrodynamics in flotation processes. In: Laskowski J (ed.) Proceedings of the 13th International Mineral Process Congress, pp. 1261-1287. Warsaw: Elsevier. Wills BA (1992) Introduction to Mineral Processing Technology, 5th edn, pp. 558...

The ability of cholinergic agonists to activate phospholipase C in bovine adrenal chromaffin cells was examined by assaying the production of inositol phosphates in cells prelabeled with [3H]inositol. We found that both nicotinic and muscarinic agonists increased the accumulation of [3H]inositol phosphates (mainly inositol monophosphate) and that the effects mediated by the two types of recepto...

We present an all atom potential energy function for the simulation of proteins and nucleic acids. This work is an extension of the CH united atom function recently presented by S. J. Weiner et al. (J . Amer. Chem. SOC., 106,765 (1984). The parameters of our function are based on calculations on ethane, propane, n-butane, dimethyl ether, methyl ethyl ether, tetrahydrofuran, imidazole, indole, d...