Monolayer techniques were used to study the interactions of various lipids (cholesterol, lysophosphatidyl choline, phosphatidal ethanolamine, phosphatidyl choline, sphingomyelin, stearic acid, and lipids extracted from plasma high density lipoproteins and very low density lipoprotein) with the lipid-free protein subunit of rat plasma high density lipoprotein and with rat plasma albumin. The pro...

Phospholipids are part of the milk fat globule membrane. Functional and technological properties make them interesting for the development of functional foods or as ingredients of technological interest. In this study an analytical procedure for determination of major phospholipids in milk (phosphatidyl Ethanolamine, phosphatidylinositol, phosphatidyl Choline, phosphatidyl Serine and sphingomye...

1. Histamine release by antigen from three sensitized rat tissues is potentiated by phosphatidyl serine (PS). The effect is greatest with isolated peritoneal cells. Phosphatidyl inositol, ethanolamine, choline and phosphatidic acid are inactive.2. PS greatly increases the rate of antigen-induced histamine release and only slightly prolongs the duration of the release process.3. PS shows a conce...

The present study employed an imaging mass spectrometry (IMS) method to evaluate the effect of dietary n – 3 fatty acids on the fatty acid composition in rat brain. Rats were divided into two groups and fed either an n – 3 fatty acid-deficient or adequate diet. We determined the decreased n – 3 fatty acids in the hippocampus of rats fed an n – 3 fatty acid-deficient diet compared to the control...

Effects of a complex polyene antibiotic, filipin, and its four components were studied upon biological (erythrocyte) and artificial (liposome) membranes in order to test the “sterol receptor” hypothesis of polyene action. The hemolytic activity of filipin complex could be removed more effectively by liposomes which had cholesterol incorporated (phosphatidyl choline (7)~dicetylphosphate (2)-chol...

Euglena gracilis was grown in the dark for 12 days in a medium containing sodium acetate1-14C. The major amount of 14C was incorporated into the neutral lipids. In these compounds myristic acid was the main fatty acid labeled. Subsequent growth in light (70 hours) in an iden­ tical medium (containing unlabeled acetate) led to a strong decrease of radiocarbon in the neutral lipids. The radioacti...

The chemical composition of the lipophylic fraction from the halophytic plant Euphorbia peplis L. was investigated. Compared to other terrestrial higher plants an increase of triacylglycerols and especially of glycolipids was observed. The main phospholipid was phosphatidyl choline, followed by almost equal concentrations of phosphatidyl ethanolamine and phosphatidyl glycerol. A relatively high...

Cells from 6 of 14 different Ly-1+ murine B cell lymphomas bound to synthetic liposomes encapsulating fluorescein. The liposomes were made from distearoyl phosphatidyl choline (DSPC), distearoyl phosphatidyl glycerol (DSPG), and cholesterol. In all cases, liposome binding was due to recognition of phosphatidyl choline by the surface IgM on the tumor cells. Liposome binding could be inhibited by...