We investigated by means of an automated ellipsometer the adsorption of prothrombin from a buffer solution by multilayers of 14:0/14:0- and 18:1/18:1-phosphatidylserine (PS) stacked on chromium slides. In this instrument thickness and refractive index of the adsorbed phospholipid and proteins are monitored continuously. Two equations are derived to relate the mass of stacked phospholipids and t...

Although long considered to be cellular debris, microparticles (MPs) are more recently considered reflective of cellular stimulation, activation, and degeneration/apoptosis. MPs that arise from the cellular components of blood and the endothelial lining of blood vessels are referred to as blood MPs and by general consensus are small (< or = 1.5 microm), expose the anionic phospholipid (PL) phos...

This corrects the article DOI: 10.1038/ncomms12862.

Because of its roles in human physiology, Aquaporin V (AQP5), a major intrinsic protein, has been a subject of many in vitro studies. In particular, a 2008 experiment produced its crystal structure at 2.0Å resolution, which is in a tetrameric conformation consisting of four protomers. Each protomer forms an amphipathic pore that is fit for water permeation. The tetramer has a pore along its qua...

The primary act of the adhesion-condensation mechanism underlying membrane fusion is considered. This act involves the formation of a close dehydrated contact between membranes and the subsequent crystallization of molecules of the external monolayers in the contact region. Crystallization associated with a decrease in the area per molecule gives rise to elastic stresses which cause a disruptio...

The ATPase activities and the amounts of individual phospholipids in E. coli wild types B163 and ML-308-225 as well as in the mutants AN120, DL54 and etc-15 have been examined. The ATPase activities and the amount of cardiolipin are higher in the stationary phase than in the log phase, whereas the amounts of phosphatidylglycerol and phosphatidylserine are lower in the stationary phase. The decr...

Nuclear medicine has become a key part of molecular imaging. In the present review article, we focus on the transbilayer phospholipids as exquisite targets for radiolabelled probes in molecular imaging. Asymmetry of phospholipid distribution is a characteristic of mammalian cell membranes. Phosphatidylcholine and sphyngomyelin cholinophospholipids are primarily located within the external leafl...

A new rapid assay for phosphatidylserine decarboxylase, which is sensitive in the nanomolar range, is described. Synthesis of radiolabeled phosphatidylserine for use as a substrate is not required, since the assay, unlike previous ones, is based on the detection of CO(2) liberated from unlabeled phosphatidylserine. The assay employs a gas chromatographic procedure for the analysis of methane fo...

The aim of this study was (1) to analy�e fresh intact or differently influenced rabbit sperm samples, using Annexin V–binding method for the detection of membrane phosphatidylserine translocation, and (2) to estimate obtained results in relation to the conceptional rate of rabbit females fertili�ed with semen from different groups. Following sample groups were designed: 1. freshly collected sem...

The electric conductance of the cation-permeable gramicidin channel in negatively charged phosphatidylserine membranes has been studied. At low electrolyte concentrations the single-channel conductance is much larger in the negatively charged membrane than in a neutral membrane. This enhancement of conductance is in agreement with theoretical expectations, although a complete description of the...