The observation of Boman and Kaletta (I, 2) that venom phosphodiesterase is capable of hydrolyzing the so called native thymus deoxyribonucleic acid has been confirmed in several laboratories (3-5). Boman (6) noticed that under appropriate conditions the reaction may be carried almost to completion. All these experiments were performed with comparatively crude preparations of phosphodiesterase....

The concentration of adenosine 3',5'-monophosphate (cyclic AMP) and the activity of adenylate cyclase were determined for the first time in conjuncation with cyclic 3',5'-nucleotide phosphodiesterase (phosphodiesterase) during the growth cycle of Tetrahymena pyriformis. High levels of cyclic AMP observed during early exponential and late stationary phases were associated with elevated adenylate...

A heat-labile inhibitor protein of adenylate cyclase (EC 4.6.1.1) and phosphodiesterase (EC 3.1.4.17) has been purified to apparent homogeneity from bovine brain cerebrum by a simple two-column procedure. The inhibitor exerts its effect on adenylate cyclase or phosphodiesterase by forming a complex with the Ca2+-dependent activator protein, thereby competing with the apoenzyme for the activator...

p-Nitrophenylphosphocholine phosphodiesterase activity was purified 5000-fold from mouse brain by treatment of membranes with Bacillus cereus phospholipase C preparation and sequential chromatographies on concanavalin A-Sepharose and CM-Sephadex columns. The phosphodiesterase (Zn(2+)-requiring) showed Km and Vmax. values of 5.5 microM and 4.2 mumol/min per mg respectively in the hydrolysis of p...

The effect of thyroid hormone on the activity of low Km cyclic AMP) phosphodiesterase of the particulate fraction from the kidney was investigated. This enzyme activity increased in the cortex from hypothyroid rats and reverted to the normal level after the subcutaneous administration of L-3,5,3'-triiodothyronine (T3) daily for 3 days. However, this hormone did not affect the enzymes from the n...

The calcium-dependent regulatory protein (CDR) purified from bovine brain was iodinated with Na[125I]I using the lactoperoxidase-glucose oxidase system. The iodinated protein retained its ability to stimulate the Ca2+-sensitive CDR-depleted cyclic nucleotide phosphodiesterase from bovine heart. Stimulation of the phosphodiesterase by 125I-CDR was Ca2+-dependent and the labeled protein had a Ka ...

Zur Therapie der idiopathischen Lungenfibrose (IPF) stehen derzeit 2 zugelassene antifibrotisch wirkende Medikamente zur Verfügung: Nintedanib und Pirfenidon. Beide können das Fortschreiten Fibrose lediglich verlangsamen. Phosphodiesterase-Hemmer (PDE-Hemmer) wirken antiinflammatorisch antifibrotisch, kommen also als Therapieoption infrage. Insbesondere Inhibitoren des PD-Subtyps 4B sind mit ve...

Reis (1) discovered 5’-nucleotidase in 1934. Shortly thereafter, Gulland and Jackson (2) showed its presence in venoms of many species of snake. Although several methods of separation of 5’-nucleotidase from phosphodiesterase of venom have been proposed (3-7), the available preparations of 5’-nucleotidase are still rather crude (8-10). Two reasons prompted us to purify venom 5’-nucleotidase. Fi...

This study tested the hypothesis that regulation of 3',5'-cAMP levels in the kidney vasculature is abnormal in spontaneously hypertensive rats. In isolated, perfused kidneys from adult rats (16 weeks of age), isoproterenol similarly increased renal venous 3',5'-cAMP secretion from kidneys of hypertensive versus normotensive Wistar-Kyoto rats. However, a broad-spectrum phosphodiesterase inhibito...