Two-photon (2P) excited fluorescence microscopy is particularly advantageous for imaging biological specimens since it offers reduced scattering, deep sample penetration, and intrinsic confocality when excited with point excitation. Here we report the first 2-photon microscopy of individual quantum dots in biological environment. Near-complete suppression of blinking and of photobleaching was a...

Spectral imaging permits two-dimensional mapping of the backscattering properties of biological systems. Such mapping requires broadband illumination of the entire area of interest. However, imaging of turbid biological media under these conditions often involves mean photon path lengths that exceed the pixel size. Using a numerical Monte Carlo model, we have studied the effects of photon scatt...

The features of electromagnetic processes are considered which connected with finite size of space region in which final particles (photon, electron-positron pair) are formed. The longitudinal dimension of the region is known as the formation length. If some external agent is acting on an electron while traveling this distance the emission process can be disrupted. There are different agents: m...

We have recently introduced the idea of making use of three-photon positron annihilations in positron emission tomography. In this paper the basic characteristics of the three-gamma imaging in PET are studied by means of Monte Carlo simulations and analytical computations. Two typical configurations of human and small animal scanners are considered. Three-photon imaging requires high energy res...

Entangled-photon pairs ~biphotons! generated by spontaneous optical parametric down-conversion exhibit a number of properties that are analogous to those of ordinary photons generated by incoherent sources. The spatial pump-field distribution and the two-particle wave function in the biphoton case play the respective roles of the source intensity distribution and the second-order coherence func...

Background: The present work aims to study the out-of-field photon calculation accuracy of a commercial treatment planning system (TPS), Oncentra, for three-dimensional conformal radiotherapy (3D-CRT) and intensity modulated radiation therapy (IMRT) treatment techniques from Elekta Synergy medical linear accelerator. Materials and Methods: Accuracy of individual out-of-field dose components was...

introduction:  skin  sparing  is  one  of  the  most  desirable  characteristics  of  high  energy  photon  beams. however, the photons emerging from the target of linacs are contaminated by secondary  electrons  as  a  result  of  their  interactions  with  air,  collimators,  flattening  filter  and  any  other  objects in their path. this phenomenon tends to increase the skin dose received b...

The EBT Gafchromic radiochromic film is a relatively new product designed specifically for dosimetry in radiation therapy. Due to the weak dependence of its response on the photon energy (variations are below 10% in the 50 kVp-10 MVp range), the film is ideal for dosimetry when the photon energy spectrum may be changing or unknown. In order to convert a map of optical densities into a map of ab...