Forty-two, one-day-old specific pathogen-free (SPF) chickens were randomly divided into two groups. On 21st day of study, experimental group was infected intraocularly with 1×106 EID50 of H9N2 and intratracheally with 1× 1010 CFU of Ornithobacterium rhinotracheale (ORT). Control group was inoculated with sterile PBS intraocularly. The samples from various tissues were collected on days 2, 4, 6,...

Insulin secretion is controlled by the beta cell's metabolic state, and the ability of the secretory granules to undergo exocytosis increases during glucose stimulation in a membrane potential-independent fashion. Here, we demonstrate that exocytosis of insulin-containing secretory granules depends on phosphatidylinositol 4-kinase (PI 4-kinase) activity and that inhibition of this enzyme suppre...

The role of specific membrane lipids in transport between endoplasmic reticulum (ER) and Golgi compartments is poorly understood. Using cell-free assays that measure stages in ER-to-Golgi transport, we screened a variety of enzyme inhibitors, lipid-modifying enzymes, and lipid ligands to investigate requirements in yeast. The pleckstrin homology (PH) domain of human Fapp1, which binds phosphati...

During exocytosis, certain phospholipids may act as regulators of secretion. Here, we used several independent approaches to perturb the phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] level in bovine chromaffin cells to investigate how changes of plasmalemmal PI(4,5)P2 affect secretion. Membrane levels of PI(4,5)P2 were estimated by analyzing images of lawns of plasma membranes labeled with ...

Absolute rate constants for hydrogen abstraction from 4-methylphenol (para-cresol) by the lowest triplet states of 24 aromatic ketones have been determined in acetonitrile solution at 23 degrees C, and the results combined with previously reported data for roughly a dozen other compounds under identical conditions. The ketones studied include various ring-substituted benzophenones and acetophen...

TRPV6 is a member of the transient receptor potential superfamily of ion channels that facilitates Ca(2+) absorption in the intestines. These channels display high selectivity for Ca(2+), but in the absence of divalent cations they also conduct monovalent ions. TRPV6 channels have been shown to be inactivated by increased cytoplasmic Ca(2+) concentrations. Here we studied the mechanism of this ...

The distortion on the intermittency signal, due to detection efficiency and to the presence of pre–equilibrium emitted particles, is studied in a schematic model of nuclear multi-fragmentation. The source of the intermittency signal is modeled with a percolating system. The efficiency is schematized by a simple function of the fragment size, and the presence of pre–equilibrium particles is simu...

The high facioselectivity in the epoxidation of 4-deoxypentenosides (4-DPs) by dimethyldioxirane (DMDO) correlates with a stereoelectronic bias in the 4-DPs' ground-state conformations, as elucidated by polarized-pi frontier molecular orbital (PPFMO) analysis.

Despite the central bioenergetic importance of inorganic phosphate (Pi), little is known about the control of Pi metabolism in skeletal muscle, where resting [Pi] is maintained at 2-4 mM despite a highly unfavourable electrochemical gradient, and is partially regulated against changes in extracellular [Pi] [ I ,2]. We have discussed elsewhere how cellular [Pi] can be controlled by active transm...

The activity of many proteins depends on the phosphoinositide (PI) content of the membrane. E.g., dynamic changes of the concentration of PI(4,5)P2 are cellular signals that regulate ion channels. The susceptibility of a channel to such dynamics depends on its affinity for PI(4,5)P2. Yet, measuring affinities for endogenous PIs has not been possible directly, but has relied largely on the respo...