Abstract Increasing atmospheric CO 2 enhances plant biomass production and may thereby change nutrient concentrations in tissues. The objective of this study was to identify the effect elevated on grassland that have been grown for 16 years (1998–2013). at extensively managed Giessen FACE experiment, fumigated with ambient (aCO ; eCO +20%) harvested twice annually. Concentrations C, N, P, K, Ca...