The application of tissue culture techniques, particularly in the area of embryo rescue, has had a major impact on the maintenance and development of hybrid embryo from wide crosses. Embryo rescue techniques are directed towards obtaining more efficient survival of embryos in situations where very immature embryos are to be cultured. An in vitro embryo rescue culture technique has been develope...

A novel micropropagation method for pineapple (Ananas comosus L.), based on shoot elongation induced in vitro, was demonstrated for two cultivars. Decapitated in vitro plantlets were used as explants. Shoot etiolation was induced by placing explants in a Murashige and Skoog (MS) medium containing NAA (10 μM) and incubating in darkness at 28C for 30 to 40 days. The mean number of the regenerated...

Research was carried out on possibilities to improve methods commonly used in commercial potato tissue culture laboratories, predominantly those in Western Canada. The study included: (1) Photoautotrophic micropropagation, (2) Short-term, low temperature (< 12 weeks) storage of plantlets, (3) Production of microtubers (in vitro tubers) and (4) Utilization of microtubers in greenhouse production...

The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species. Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate is using in vitro culture techniques, and...

A protocol was established for mass clonal propagation of Phalaenopsis amabilis cv. 'Golden horizon' through in vitro culture of young leaf segments from mature plant. Explants were cultured on half strength Murashige and Skoog (1/2 MS) medium supplemented with N6-benzyladenine (2.0 mg l-1), a-naphthaleneaceetic acid (0.5 mg l-1), 2% (w/v) sucrose, 10% (v/v) coconut water, 2 g l-1 peptone and 1...

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficient in vitro regeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three type...

Plantlets of Populus yunnanensis Dode were examined in a greenhouse for 48 h to analyze their physiological and proteomic responses to sustained heat, drought, and combined heat and drought. Compared with the application of a single stress, simultaneous treatment with both stresses damaged the plantlets more heavily. The plantlets experienced two apparent response stages under sustained heat an...

Burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "B. brasilensis" species. This work assessed the ability of B. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different...

Malik A.A., Li Cui, Shuxia Zhang, Jin-feng Chen, 2011. Efficiency of SSR markers for determining the origin of melon plantlets derived through unfertilized ovary culture. Hort. Sci. (Prague), 38: 27–34. The effects of temperature pre-treatment, thidiazuron, naphthaleneacetic acid, and 6-benzylaminopurine on in vitro gynogenic plant production from un-pollinated melon (Cucumis melo L.) ovaries w...

Tissue cultured Alpinia galanga plantlets were subjected to assessment of genetic stability considering the fact that associated in vitro stress might result in breakdown of control mechanism causing instability of the genome. We have used two DNA based molecular markers to assess the genetic fidelity of in vitro regenerated Alpinia galanga L. through axillary buds from unsprouted rhizomes. The...