نتایج جستجو برای: plaque reduction neutralization test
تعداد نتایج: 1303862 فیلتر نتایج به سال:
A plaque reduction method for measuring lymphocytic choriomeningitis virus-sensitizing antibody in human serum is described. One volume of virus and one volume of serially diluted human serum were mixed and incubated for 2 h at 37 degrees C. One volume of suitably diluted anti-human immune globulin antiserum was added and incubation continued for 0-5 h. Residual infectivity was then determined ...
Because of the global spread of Zika virus, accurate and high-throughput diagnostic immunoassays are needed. We compared the sensitivity and specificity of 5 commercially available Zika virus serologic assays to the recommended protocol of Zika virus IgM-capture ELISA and plaque-reduction neutralization tests. Most commercial immunoassays showed low sensitivity, which can be increased.
We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.
Among our cohort of adolescents and young adults with perinatally acquired human immunodeficiency virus, few (17.6%) had measles protective antibodies by plaque reduction neutralization (PRN). Agreement was demonstrated between the commercial enzyme immunoassay and the PRN assay (K = 0.59 [95% confidence interval: 0.23-0.95]). Further studies are needed to understand the determinants of immunit...
Virus neutralization assays measure neutralizing antibodies in serum and plasma, the plaque reduction test (PRNT) is considered gold standard for measuring levels of these many viral diseases. We have developed procedures PRNT, microneutralization assay (MNA) pseudotyped virus (PNA) severe acute respiratory syndrome coronavirus 2. The MNA offers advantages over PRNT by reducing time, allowing i...
Dengue viruses (DENV) cause countless human deaths each year, whilst West Nile virus (WNV) has re-emerged as an important human pathogen. There are currently no WNV or DENV vaccines licensed for human use, yet vaccines exist against other flaviviruses. To investigate flavivirus cross-reactivity, sera from a human cohort with a history of vaccination against tick-borne encephalitis virus (TBEV),...
The antigenic relationship of the 31 currently recognized phlebotomus fever group viruses was examined by plaque reduction neutralization test. Although some low-level 0cross-neutralization was observed, each of the viruses was easily differentiated by this method. Rift Valley fever virus was shown to be antigenically related to Candiru, Frijoles, Karimabad and Punta Toro viruses. Naples, Tehra...
background and aims: canine distemper (cd) is a deadly infectious disease of canidae family. cd is a multi-systemic viral disease and is specified by wide range of clinical symptoms. the manifestations are not always indicative of cd, therefore a laboratory confirmation is necessary for suspected cases. materials and methods: different clinical specimens of 19 cd suspected unvaccinated dogs wer...
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