Methicillin-resistant Staphylococcus aureus (MRSA) has been listed by the World Health Organization as a priority antibiotic-resistant nosocomial pathogen in urgent need of new antimicrobials [1]. Plasmids are key factor pathology and epidemiology MRSA isolates play important roles their evolution. In our on-going efforts to better understand genetic background molecular from Terengganu, Malays...

Mutations in the gene encoding topoisomerase I of Escherichia coli were tested for their effect on plasmid recombination. Recombination was decreased 1,000-fold at 30 and 37 degrees C and occurred at approximately wild-type frequencies at 42 degrees C. The suppression of topA mutations at 42 degrees C did not appear to be a result of increased topoisomerase I activity at 42 degrees C.

Here we report the results of second interim analysis a randomized prospective multi-center clinical study aimed to evaluate safety and efficacy p62/SQTM1-encoding plasmid applied as an adjuvant chemotherapy in patients with advanced platinum-resistant ovarian cancer. P62 encoding acts classic anti-cancer DNA vaccine it also lowers chronic inflammation thus rendering tumor cells more susceptibl...

Investigation of the Frequency Plasmid-Dependent Quinolone Resistance Genes in Klebsiella Pneumoniae, Pseudomonas Aeruginosa and Escherichia Coli Isolated from Surgical Site Infections

from plasmid pSCFS1, was found downstream of the cfr gene in pMSA16. The 2848 bp fragment containing the cfr gene and its downstream pre/mob gene showed 100% nucleotide sequence identity to the corresponding parts in plasmids pBS-01 and pBS-02. In conclusion, this is the first report describing the presence of the cfr gene in a bovine MRSA ST9 strain. Moreover, this is also the first report of ...

The distribution of the IncFI basic replicons among IncFIV plasmids was assessed by DNA hybridization. In addition these and 20 other plasmids from 16 incompatibility groups were screened for the presence of IncIV, an incompatibility determinant recently found on the IncFIV plasmid R124. The IncIV determinant was found commonly but not universally among the IncFIV plasmids. It was also detected...

Using plasmids capable of undergoing intramolecular recombination, we have compared the rates and the molecular outcomes of recombination events in a wild-type and a rad52 strain of Saccharomyces cerevisiae. The plasmids contain his3 heteroalleles oriented in either an inverted or a direct repeat. Inverted repeat plasmids recombine approximately 20-fold less frequently in the mutant than in the...

The biodegradation of secondary amines is particularly important due to their propensity for conversion either chemically or microbiologically to N-nitrosamines which are potent carcinogens. In this research, a weak Gram-positive organism was isolated from river and identified as Mycobacterium. This Mycobacterium grows slowly and effectively utilizes piperazine as the sole source of organic, c...