The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule. It is used to determine whether a specific DNA sequence is present in the sample; and if it is present, the number of copies in the sample. It is the real-t...

bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal flora of the vagina, which leads to the elimination of lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. this disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...

ABSTRACT Diversity in host range, pathogenicity, phenotypic characteristics, repetitive extragenic palindromic polymerase chain reaction (rep-PCR) profiles, and sequence of the 16S-23S rDNA spacer region was examined among 44 Xanthomonas strains isolated from lettuce. Forty-two of the strains were divided into two groups, designated A and B. Seventy percent were Group A, and most of the remaini...

background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr.  materials and methods: genomic dna from bacillus thermoca...

A Short History of the Polymerase Chain Reaction. In the 1950s, the discovery of PCR is the subject of claim and counterclaim that has yet to be.Methods Mol Biol. A short history of the polymerase chain reaction. Author information: 1Division of Cancer and.A Short History of the Polymerase Chain Reaction. Full Text, Download PDF 103K. And although this does risk overstating the impact of PCR ou...

The purpose of this article is to review the status of DNA assays used for the detection, identification, and individualization of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Brucella abortus. These select agent microorganisms are historically significant as they have either been used or experimented with as a bioweapon or as a terrorist agent and are t...

Genotypes of Iranian Zel sheep for Calpastatin(CAST) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods and for Calpain(CAPN) locus by PCR-SSCP. Blood samples were collected from 200 purebred Zel sheep of Zel Breeding Station located in Golestan province i...

Brocchi M., Ferreira A., Lancellotti M., Stehling E.G., Campos T.A., Nakazato G., Pestana de Castro A.F. & Silveira W.D. 2006. Typing of avian pathogenic Escherichia coli strains by REPPCR. Pesquisa Veterinária Brasileira 26(2):69-73. Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade de Campinas, Cx. Postal 6109, Campinas, SP 13081-862, Brazil. E-mail: wds@unicamp....

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