A specific, 0.1-0.3-microm large ribonucleoprotein complex consisting of a central core with stalklike extensions on top of which 280-320-A ribonucleoprotein particles are situated is found in an experimentally activated chromosome region, 2-48C, of the polytene chromosomes of Drosophila hydei. Alkaline hydrolysis, RNAse digestion, and uranyl-EDTA-lead staining indicated the ribonucleoprotein c...

In situ hybridization is particularly appropriate for mapping specific DNA sequences on polytene chromosomes of Drosophila and other dipterans. This technique is based on the recognition and binding of one labeled sequence (the probe) to homologous sequences on chromosomes fixed on a microscope slide. The probes are labeled with biotin or other nonradioactive products, and the probe signal can ...

The African chironomid Polypedilumvanderplanki Hinton, 1951 is the only chironomid able to withstand almost complete desiccation in an ametabolic state known as anhydrobiosis. The karyotypes of two allopatric populations of this anhydrobiotic chironomid, one from Nigeria and another from Malawi, were described according to the polytene giant chromosomes. The karyotype from the Nigerian populati...

Drosophila has long been a favorite model system for studying the relationship between chromatin structure and gene regulation due to the cytological advantages provided by the giant salivary gland polytene chromosomes of third instar larvae. In this tissue the chromosomes undergo many rounds of replication in the absence of cell division giving rise to approximately 1000 copies. The DNA remain...

eosinophil autofluorescence. J. Leukoc. Biol. 51:172-175. 12.Nokubo, M., M. Ohta, K. Kitani and I. Z.Nagy. Identification of protein-bound riboflavin in rat hepatocyte plasma membrane as a source of autofluorescence. Biochim. Biophys. Acta 981:303-308. 13.Olson, K.R., J.R. McIntosh and J.B. Olmsted. 1995. Analysis of MAP4 function in living cells using green fluorescent protein (GFP) chimeras. ...

DNA replication of Chironomus polytene chromosomes was investigated The results show that during treatment with ethanol there is an accumulation of newly synthesized DNA with the size expected of replicons. These and previous results indicate that it is possible that in polytene chromosomes the termination of replication and merging of adjacent replicons is more sensitive to damage than in init...

The SuUR (suppressor of underreplication) gene controls late replication and underreplication of DNA in Drosophila melanogaster polytene chromosomes: its mutation suppresses DNA underreplication whereas additional doses of the normal allele strongly enhances underreplication. The SuUR protein is localized in late replicating and underreplicating regions. The N-terminal part of the SuUR protein ...

In polytene chromosomes of Drosophila melanogaster, regions of pericentric heterochromatin coalesce to form a compact chromocenter and are highly underreplicated. Focusing on study of X chromosome heterochromatin, we demonstrate that loss of either SU(VAR)3-9 histone methyltransferase activity or HP1 protein differentially affects the compaction of different pericentric regions. Using a set of ...

Drosophila polytene chromosomes are compacted into a series of bands and interbands. Z4 is a protein to keep this pattern of polytene chromosomes, since Z4 mutant larvae show a decompaction of chromosomes and a loss of banding pattern (Eggert et al., 2004). By coimmuno-precipitation, we identified a chromodomain protein, which we named Chriz, for chromodomain protein interacting with Z4 (Gortch...

Preface ..............................................................................................................v Contributors ..................................................................................................... ix Color Plates ................................................................................................... xiii 1. The Chromosomes of Drosophila melanoga...