BACKGROUND Metabolic engineering aims at channeling the metabolic fluxes towards a desired compound. An important strategy to achieve this is the modification of the expression level of specific genes. Several methods for the modification or the replacement of promoters have been proposed, but most of them involve time-consuming screening steps. We describe here a novel optimized method for the...

Leishmania parasites include important pathogens and model organisms are even used for the production of recombinant proteins. However, functional genomics characterization essential genes often limited in because low-throughput technologies gene disruption or tagging absence components RNA interference. Here, we tested T7 polymerase-dependent CRISPR-Cas9 system by Beneke et al. glmS ribozyme-b...

Uncovering the underlying regulatory mechanism is an important research content in bioinformatics. Due to the single data source providing only regulation between partial and noisy information, so a variety of biological data integration methods must be used to get more reliable networks inference. This paper proposes a novel integrated method by combing ChIPchip data, transcription factor knoc...

The article discusses the possibility of using glycerol as an additive to engine fuel in order reduce tendency combustion knock, and thus increase octane number a given fuel. Experimental tests were carried out on UIT-85 research with variable compression ratio from eight eleven test intensity knock. completely renewable fuel—the blend butanol 25 75%, respectively—was tested. A comparative anal...

thermophysical properties of fluorine are interesting because of its applications in different areas of technologyand industry. the computations of thermodynamic properties were performed with the gma equation of stateunder critical conditions. the present work reports calculations of density, volumetric, and thermodynamicproperties of liquid fluorine on extended ranges of pressure and temperat...

the current study aims to investigate the relationship between stock liquidity risk and financial information quality criteria (i.e. the timely dividends announcement, accruals quality and the percentage of profitability prediction error) of companies listed on the tehran stock exchange. for this purpose, 148 cases of data from listed companies, collected from 2007 to 2012, were employed in ord...