Restriction enzymes are well known as reagents widely used by molecular biologists for genetic manipulation and analysis, but these reagents represent only one class (type II) of a wider range of enzymes that recognize specific nucleotide sequences in DNA molecules and detect the provenance of the DNA on the basis of specific modifications to their target sequence. Type I restriction and modifi...

Streptomyces lividans DNA contains a modification which makes it susceptible to double-strand cleavage during electrophoresis in buffers contaminated with ferrous iron (which may be present in some batches of EDTA). The cleavage of the DNA is site-specific and the average fragment size resulting from limit digestion of total S. lividans DNA is about 6kb. DNA from Streptomyces coelicolor A3(2) a...

5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation sequencing.

BACKGROUND There is an increasing interest among nutritional researchers to perform lifestyle and nutritional intervention studies in a home setting instead of testing subjects in a clinical unit. The term used in other disciplines is 'ecological validity' stressing a realistic situation. This becomes more and more feasible because devices and self-tests that enable such studies are more common...

A substrate-triggered technique is proposed to improve electrostatic discharge (ESD) protection efficiency of ESD protection circuits without extra salicide blocking and ESD-implantation process modifications in a salicided shallow-trench-isolation CMOS process. By using the layout technique, the whole ESD protection circuit can be merged into a compact device structure to enhance the substrate...

MODIFICATION OF DEPOSITION PROCESS PARAMETERS FOR UNIFORM INDIUM LAYER DEPOSITION Isaac Butt Old Dominion University, 2017 Director: Dr. Sylvain X. Marsillac The need for more efficient light to energy converting cells has long been a subject of research and development. With abundant availability of solar energy that the earth receives, the photovoltaic industry has sought materials that could...

The development of high quality large-scale software systems within schedule and budget constraints is a formidable software engineering challenge. The modification of these systems to incorporate new and changing capabilities poses an even greater challenge. This modification activity must be performed without adversely affecting the quality of the existing system. Unfortunately, this objectiv...

grafting polymerization was a valuable method for the modification of the chemical and physical properties of polymer surfaces. in this paper, the grafting of cationic vinyl monomer with quaternary ammonium groups, methacryloxyethyltrimethyl ammonium chloride (dmc), onto the isotactic polypropylene (ipp) granule was performed by suspension solid-state grafting process. the technique offers new ...

In this article we examine four objections to the genetic modification of human beings: the freedom argument, the giftedness argument, the authenticity argument, and the uniqueness argument. We then demonstrate that each of these arguments against genetic modification assumes a strong version of genetic determinism. Since these strong deterministic assumptions are false, the arguments against g...

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