DOG(R)1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOG(R)1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l(-1) 2-deoxyglucose (2-DOG) as the ...

A method has been developed for plant regeneration by encapsulation of somatic embryos obtained from callus cultures derived from semi-mature cotyledon explants of Dalbergia sissoo Roxb. (family Fabaceae). Embryogenic callus was developed from cotyledon pieces on Murashige and Skoog (1962) medium supplemented with 9.04 μM 2,4dichlorophenoxyacetic acid and 0.46 μM kinetin. The somatic embryos we...

An efficient in vitro regeneration protocol was established for Swertia chirayita. The present study describes the regeneration of S. chirayita from shoot tip explants collected from wild grown plantlets. The highest number of shoots (26.10 ± 0.73) were obtained on Murashige and Skoog basal medium supplemented with 6-benzyladenine (BA) (0.5mg/l) in combination with gibberellic acid (GA3) (1.0 m...

strawberry is a valuable, nutritious, and economically important fruit all over the world including bangladesh. therefore, there is a demand to develop a suitable variety of strawberry. for this purpose, leaf explants from in vitro grown strawberry plantlets were cultured onto ms medium supplemented with different concentrations and combinations of 2,4-d, naa and ba for callus induction. the mo...

To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of t...

Cattleya intermedia is an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium an...

Physiological characteristics of in vitro and field cultivated Leucojum aestivum l. Plants stanilova m. summary. A living collection of Leucojum aestivum L. (Amaryllidaceae) was established at the Institute of Botany, Sofia, in 2001. In vitro cultures originating from a single mother bulb have been maintained since 2003 on MS-based agar-solidified medium supplemented with 30 g L-1 sucrose, 2 mg...

The clonal propagation of T. cacao by somatic embryogenesis (SE) is a promising approach to multiply elite genotypes. Assessing fidelity in plants regenerated from embryos the first step toward ensuring genetic uniformity mass production planting material. This study assessed stability plantlets propagated SE and conventional grafting for genotypes CCN51 TSH565 using 13 SSR. leaves vitro (IVL) ...

Somatic embryogenesis is one of good examples of the basic research for plant embryo development as well as an important technique for plant biotechnology such as medicinally important plants. Single embryos develop into normal plantlets with shoots and roots. Therefore, direct single embryogenesis derived from single cells is highly important for normal plant regeneration. Here we demonstrate ...

Hairy root cultures of Mitragyna speciosa were established by infection of Agrobacterium rhizogenes ATCC 15834 and maintained in McCown woody plant medium (WPM) supplemented with 0.5 mg/1 naphthaleneacetic acid. The hairy roots were identified for the rooting genes loci of rolA and rolB by polymerase chain reaction. For studying the secondary metabolite production, the n-hexane extract of the h...