Prostate-specific antigen (PSA, hK3) is a diagnostic marker for prostatic cancer but lacks the specificity to sufficiently distinguish between prostatic cancer and benign prostatic hyperplasia (BPH). Human glandular kallikrein 2 (hK2) has been proposed as a potential diagnostic marker for prostate cancer that could complement the current PSA test. Recently we demonstrated that proPSA is present...

Endogenous androgen levels of prostatic tissues in patients with benign prostatic hypertrophy (BPH) and untreated prostatic cancer (PC) were determined by radioimmunoassay using sephadex LH-20 column chromatography. By this assay procedure, it was possible to measure tissue androgen levels with only 30 approximately 40 mg weight of tissues. In BPH (n=20) the concentrations of steroids (mean +/-...

The ƒÁ-Seminoprotein (ƒÁ-Sm) and the Prostatic Acid Phosphatase (PAP) have recently been studied as useful tumor markers for prostatic cancer. Both PAP and ƒÁ-Sm in patients with prostatic cancer have been assayed and the data relevant to the usefulness of ƒÁ-Sm as well as PAP have been analyzed. The results obtained are as follows. A total of 89 cases with prostatic cancer, Benign Prostatic Hy...

To study the mechanism of regression of human prostatic cancer following androgen ablation, the androgen-responsive PC-82 human pros tatic adenocarcinoma xenograft was used as a model system. Castration of male nude mice bearing PC-82 xenografts results in a 50' i tumor regression by 2 wk following androgen ablation. This regression is due to a sequence of biochemical and morphological events t...

498 CLINICALCHEMISTRY, Vol. 31, No. 3, 1985 Chu TM. Purification of a human prostate specific antigen. Invest Urol 17, 159-163 (1979). 3. Ablin RJ. Immunochemical identification of prostatic tissue-specific acid phosphatase. Glin Ghem 19, 786 (1973). Letter. 4. Ablin RJ. Prostate-specific acid phosphatase. Clin Ghem 26, 1632-1633 (1980). Letter. 5. Ablin RJ, Bronson P, Soanes WA, Witebsky E. Ti...

We have used microcell fusion-mediated chromosomal transfer to introduce normal human chromosomes into highly metastatic rodent prostatic cancer cells to map the location of a metastasis suppressor gene(s). Using this approach, several chromosomal regions have been identified that harbor such metastatic suppressor genes, including human chromosome 11 between p11.2-13 (T. Ichikawa et al., Cancer...

background: prostate specific antigen (psa) is an important laboratory marker for diagnosis of prostatic cancer. thus, development of diagnostic tools specific for psa plays an important role in screening, monitoring and early diagnosis of prostate cancer. in this paper, the production and characterization of a panel of murine monoclonal antibodies (mabs) against psa have been presented.  metho...

Prostatic diseases are a common health problem among males in Western countries, and include chronic prostatic diseases, which have an unclear pathogenesis and few treatment options. In vitro and in vivo studies describe oxidative stress as a major pathway involved in the occurrence of benign prostatic hyperplasia, prostatic cancer and chronic prostatitis. Thus, the oxidative stress cascade is ...

We present a systematic analysis of human prostatic fluid with two-dimensional gel electrophoresis (the ISO-DALT system) and a characterization of normal and disease-related protein patterns. A reference map for prostatic fluid proteins was established by analysis of pooled prostatic fluids from 80 men (age less than or equal to 50 years) without prostatic lesions. Proteins in prostatic fluid t...

thirty cases of benign prostatic hyperplasia and 30 cases of prostatic adenocarcinoma were selected and stained by agnor (argyrophilic staining of the nucleolar organizer region) method. specimens were obtained by either prostatectomy (open!tijr) or needle biopsy of the prostate. in this study, the sections of prostatic adenocarcinoma were reviewed. 6 cases were diagnosed as well-differentiated...