نتایج جستجو برای: protamine damage

تعداد نتایج: 228243  

Journal: :The Journal of clinical investigation 1974
R M Krauss R I Levy D S Fredrickson

An assay has been developed for specific measurement of two different lipase activities in postheparin plasma. Lipoprotein lipase, derived from extrahepatic sources, is measured as protamine-inactivated lipase activity; hepatic lipase activity is protamine-resistant under the conditions of this assay. In 100 normal subjects, both enzyme activities were noted to be related to age and sex. Protam...

2005
Edward Lowenstein Martin L. Ogletree Elizabeth M. Greene Dwight R. Robinson Karen Hartl Warren M. Zapol

We used competitive thromboxane A2-prostaglandin endoperoxide receptor blockade (SQ 30,741) as a probe to evaluate the role of thromboxane in ovine pulmonary vasoconstriction associated with protamine reversal of heparin anticoagulation. Control heparin-protamine reactions induced rapid release of thromboxane into arterial plasma (more than 1 ng/ml plasma), a 2.5-fold increase of pulmonary arte...

Journal: :cell journal 0

introduction: the aim of this study was to evaluate the effect of sperm protamine deficiency on fertilization and embryo development post icsi. material and methods: semen samples from 27 patients candidate for icsi were assessed for semen parameters. sperm processing was carried out using pure sperm and percentage of protamine deficient sperm was assessed by chromomycin a3 post processing. cor...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1987
J J Peschon R R Behringer R L Brinster R D Palmiter

Protamines are abundant basic proteins involved in the condensation of sperm chromatin. In the mouse, protamine genes are transcribed postmeiotically in round spermatids. We have cloned and sequenced the mouse protamine 1 gene. Ten lines of transgenic mice harboring marked protamine 1 sequences were generated by microinjection of fertilized eggs. Transcription of the transgene is restricted to ...

Journal: :Human reproduction 2014
L Simon L Liu K Murphy S Ge J Hotaling K I Aston B Emery D T Carrell

STUDY QUESTION Is there an association between sperm DNA damage, measured by three different assays, sperm nuclear protein content and clinical outcomes in assisted reproduction treatment (ART)? SUMMARY ANSWER Sperm DNA damage measured by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) and the Comet assay were significantly associated with ART outcomes in our sin...

Journal: :Analytical chemistry 2008
Katherine Fordyce Alexey Shvarev

We report here on improved solid-contact electrochemical polyion sensors for the detection of polyion protamine. The polymeric membrane sensors were fabricated with a conducting polymer as an ion-electron transduction layer. We observed that decreasing the magnitude of the applied current pulse caused a significant improvement of the sensor sensitivity to low protamine levels. The protamine sen...

Journal: :Molecular biology and evolution 2000
A P Rooney J Zhang M Nei

Protamines are small, highly basic DNA-binding proteins found in the sperm of animals. Interestingly, the proportion of arginine residues in one type of protamine, protamine P1, is about 50% in mammals. Upon closer examination, it was found that both the total number of amino acids and the positions of arginine residues have changed considerably during the course of mammalian evolution. This ev...

2012
Agnes Nemeth Maria Ablonczy Palma Sillo Renata Kun

comparison with the negative control was scored as positive. Furthermore, in vitro allergy testing using the Phadia CAP system for specific IgE against shrimp, fish-mix, rainbow trout, rCyp p 1 and protamine sulfate was performed. Results: Skin prick tests were positive to shrimp, fish-mix, mackerel, salmon and protamine sulfate. Intradermal testing with protamine showed a wheal diameter of 7 m...

2015
Salvatore Suelzu Andrea Cossu Giovanni Pala Michele Portoghese Valeria Columbanu Gabriele Sales Luigi Solinas Luca Brazzi

INTRODUCTION Currently, a dose of protamine equal to 1 mg for each 100 units of heparin given is used to reverse the residual heparin activity following off-pump coronary artery bypass. We hypothesized that a 1:1 ratio (ratio of protamine to heparin) could be higher than necessary inducing post-operative disturbance of hemostasis. METHODS Between January and March 2014 in 9 patients undergoin...

Journal: :Fertility and sterility 2014
Bolan Yu Yanbin Qi Dan Liu Xingcheng Gao Hui Chen Chuan Bai Zhaofeng Huang

OBJECTIVE To investigate the association between smoking, semen quality, and the histone-to-protamine transition ratio in mature sperm. DESIGN Biochemical and molecular analysis in human samples and a cell line. SETTING Andrology laboratory in a university-affiliated hospital. PATIENT(S) Semen samples from 147 heavy smokers and 175 nonsmokers receiving infertility treatment. INTERVENTIO...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید