Background: The hyaluronidases are the enzymes hydrolyze β-1, 4 glycosidic linkage of hyaluronan. Hyaluronan is a polymer consisting of a repeating disaccharide unit found in cumulus ovuforus complex, semen liquid and other tissue. Addition to hydrolyzing the hyaluronan, hyaluronidase can penetrate through the cumulus cells layer that surrounds the oocyte, thus it terms spreading factor. Moreov...

The polymerase chain reaction (PCR) was used to amplify a 578 bp fragment of the poxvirus 4b core protein. Avipoxvirus (APV) specific DNA was detected in all 10 different isolates (each of which had been isolated from an epidemic) isolated from chicken; canary and mynah were collected from Tehran province. Sequencing was performed for 2 isolates as representative and the nucleotide sequence sho...

Background: Glutaminase (EC 3.5.1.2) catalyzes the hydrolytic degradation of L-glutamine to L-glutamic acid and has been introduced for cancer therapy in recent years. The present study was an in silico analysis of glutaminase to further elucidate its structure and physicochemical properties.Methods: Forty glutaminase protein sequences from different species of Escherichia and Bacillus obtained...

Brief summary: This study reveals a Deep Learning method, ?RoseTTA fold?, based on DeepMind?s Alphafold2 framework, to predict 3-dimensional protein structures from 1-dimensional sequence information and generate models of protein?protein complexes with high accuracy.

Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was perform...