The present study was conducted to study the effect of protected-glutamine (Gln) supplementation on dry matter intake (DMI), milk yield (MY) and composition, somatic cell counts (SCC) and blood parameters in fresh cows. Forty Holstein cows at zero day of parturition (calving day = day 0) were divided into four groups (n=10), and fed (ad libitum) with one of the diets including: basal d...

Abstract Buffalo milk production is an activity that has grown in recent years, mainly due to the physicochemical characteristics found this type of milk, which second most produced world. The objective study was implement a milking system and monitor identity quality standards buffalo at Experimental Agronomic Station Federal University Rio Grande do Sul. Samples were collected fortnightly bet...

BACKGROUND Gyr cows are well adapted to tropical conditions, resistant to some tropical diseases and have satisfactory milk production. However, Gyr dairy herds have a high prevalence of subclinical mastitis, which negatively affects their milk yield and composition. The objectives of this study were (i) to evaluate the effects of seasonality, mammary quarter location (rear x front), mastitis-c...

In the past decade, a global demand for products from organic agriculture has increased rapidly. Milk quality is of major interest for all parties. Therefore, the objective of this study was to compare cow performance and product quality in conventional and organic system. Twenty Holstein dairy cows were allotted to one of 2 diet groups, which including: a conventional diet (CON), and an organi...

Background: Design of experiments is a rapid and cost-effective approach for optimization of recombinant protein production process. In our previous study, we generated a potent dual-acting fusion protein, anti-CD22 scFv-apoptin, to target B-cell malignant cell lines. In the present investigation, we report the effect of different variables on the expression levels of this fusion protein. Metho...

Pure Holstein (HO, n=430), crosses between Swedish Red and HO (SRxHO, n=41), Montbeliarde and HO (MOxHO, n=18), and MO and SRxHO (MOxSH, n=53) were compared for milk, fat and protein yield, fat and protein percentage, somatic cell count (SCC), milking time (MT), and age at fi rst and second calving. A total of 180,933 test-day information for milk yield and MT, and 5,249 for fat and protein per...

Lactoferrin (Lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. Therefore, the Lf has a major role in bovine mammary gland defense during mastitis. In this study, 5’-flanking region of bovine Lf gene containing three single nucleotide polymorphisms (SNPs) at positions −602 (T/G), −600 (A/G) ...

Lactoferrin (Lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. Therefore, the Lf has a major role in bovine mammary gland defense during mastitis. In this study, 5’-flanking region of bovine Lf gene containing three single nucleotide polymorphisms (SNPs) at positions −602 (T/G), −600 (A/G) ...