Male Sprague-Dawley rats that received 60% total body surface, full-thickness, scald burns on the dorsum and abdomen were used in this study. Neutral proteinase and Ca2+-activated neutral proteinase activities were measured in gastrocnemius and soleus muscles at 3 and 21 days after the thermal injury. Neutral proteinase activity decreased significantly in the soleus (50%) and gastrocnemius (46%...

A protein inhibitor of the Ca2+-dependent proteinase has been purified from bovine cardiac muscle by using the following steps in succession: salting out 17,600 X gmax supernatants from muscle homogenates in 50 mM Tris acetate, pH 7.5, 4 mM EDTA between 25 and 65% ammonium sulfate saturation; eluting between 25 and 120 mM KCl from a DEAE-cellulose column at pH 7.5; salting out between 30 and 60...

Proteolytic activity, acid phosphatase activity and fructose concentration were measured in seminal plasma from 205 human semen samples. Certain routine sperm characteristics were also determined. High fibrinolytic activity was found and TAME-esterase and proteinase activity was also present in all specimens. The activity of all the proteolytic enzymes correlated positively with the acid phosph...

A serine proteinase from roots of Taraxacum officinale Webb S. L. was isolated by affinity chromatography and gel-filtration on Superose 6R using FPLC. The enzyme is a 67-kD glycoprotein containing 54% carbohydrate which we have named taraxalisin. The substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin B-chain is comparable with that of cucumisin from Cucumis mel...

The leaves of Phaseolus vulgaris L. cv Greensleeves contain an endopeptidase with a pH optimum of 9.0 and an isoelectric point between 10.0 and 10.5. This endopeptidase is the only abundant Azocoll-digesting proteinase in the leaves. The activity of this enzyme is highest in immature leaves and declines as the leaf matures and senesces. Enzymically isolated protoplasts contain very little of th...

The evidence demonstrating excessive proteinase activity in states of shock is reviewed. The capacity of extrinsic inhibitors to control such activity is indicated and the literature showing the value of inhibitors in treating shock is summarized. Three principles to be observed in the use of exogenous inhibitors are proposed. PROTEINASE is the generic term for proteolytic enzymes. Proteolysis ...

BACKGROUND Proteinase activities are often measured using chromogenic substrates that are much smaller than physiological substrates. METHODS The hydrodynamic size of macromolecular substrates (macrosubstrates) prepared by linking small chromogenic substrates to polyethylene glycol was determined by gel filtration. Efficiency of macrosubstrate cleavage by proteinases and alpha(2)-macroglobuli...

The proteinase activity of strains of group A streptococci isolated from patients in acute and convalescent phases of illness was found to be remarkably constant. Sulfadiazine therapy of patients did not affect proteinase production by the infecting streptococci. The ability to produce streptococcal proteinase would appear to be related to the strain and not necessarily to the serological type....

Ovalbumin is partially homologous in sequence with the proteinase inhibitors alpha 1-proteinase inhibitor and anti-thrombin III. The region of sequence in ovalbumin which corresponds to the reactive sites of these proteinase inhibitors is susceptible to attack by subtilisin, elastase, thermolysin, bromelain, and Bacillus cereus protease. The esterase activity of elastase is not inhibited by ova...

Ca(2+)-activated neutral proteinase was purified from rabbit skeletal muscle by a method involving DEAE-Sephacel chromatography, affinity chromatography on organomercurial-Sepharose and gel filtration on Sephacryl S-200 and Sephadex G-150. The SDS (sodium dodecyl sulphate)/polyacrylamide-gel-electrophoresis data show that the purified enzyme contains only one polypeptide chain of mol.wt. 73000....