Cilia are centriole-based structures stretching out from cells into the extracellular environment increasing the area of contact. Both primary and motile cilia function as sensory organelles, playing a key role in transducing extracellular stimuli, and common intraflagellar transport mechanisms have shown that signaling components such as ion channels are inserted into the ciliary membrane [1–5...

Purpose. To present a case of a keratoconic patient who underwent epithelial removal with transepithelial phototherapeutic keratectomy (t-PTK) using a 213-nm solid-state laser system followed by corneal collagen cross-linking (CXL) with riboflavin and ultraviolet-A (UVA) irradiation. Methods. Case report. Results. A twenty-four-year-old male with keratoconus underwent CXL treatment after epithe...

We used the patch-clamp technique to study the effect of H(2)O(2) on the apical ROMK-like small-conductance K (SK) channel in the cortical collecting duct (CCD). The addition of H(2)O(2) decreased the activity of the SK channels and the inhibitory effect of H(2)O(2) was larger in the CCD from rats on a K-deficient diet than that from rats on a normal-K or a high-K diet. However, application of ...

Kinetochore microtubules (kMts) are a subset of spindle microtubules that bind directly to the kinetochore to form the kinetochore fiber (K-fiber). The K-fiber in turn interacts with the kinetochore to produce chromosome motion toward the attached spindle pole. We have examined K-fiber maturation in PtK 1 cells using same-cell video light microscopy/serial section EM. During congression, the ki...

Small cell lung cancer (SCLC) cell growth is sustained by multiple autocrine and paracrine growth loops involving neuropeptides. The bombesin family of peptides are autocrine growth factors in H345 SCLC cells and provide a paradigm for the study of growth factors and mitogenic signaling in SCLC cells. We show that bombesin (and other neuropeptides) stimulates protein tyrosine phosphorylation (p...

Protein tyrosine kinase (PTK) mediated the tyrosine phosphorylation modification of neuronal receptors and ion channels. Whether such modification resulted in changes of physiological functions was not sufficiently studied. In this study we examined whether the hypoxic respiratory response-which is the enhancement of breathing in hypoxic environment could be affected by the inhibition of PTK at...

It has been suggested that protein tyrosine kinase (PTK) activity can directly regulate cardiac L-type Ca(2+) channels. This conclusion is based to a large extent on the observation that the PTK inhibitor genistein can inhibit the cardiac L-type Ca(2+) current. The purpose of the present study was to determine whether the ability of genistein to inhibit cardiac L-type Ca(2+) channel activity is...

The K-LISATM PTK Screening Kit is a colorimetric kit in a 96-well plate format, which provides a rapid and sensitive method for detection of protein tyrosine kinase (PTK) activity in purified or partially purified kinase preparations, cell lysates and tissue extracts. The kit features a multiplex array of three synthetic, random polypeptide substrates of glutamic acid, alanine and tyrosine, whi...