نتایج جستجو برای: pyrosequencing
تعداد نتایج: 4566 فیلتر نتایج به سال:
aim : to evaluate the association of dnmt1 and mgmt amino acid substitution polymorphisms and colorectal cancer in iranian population. background : the mgmt and dnmt1 are two important methyltransferase enzymes. amino acid substitution polymorphisms in mgmt and dnmt1 genes may be associated with the genetic susceptibility to sporadic colorectal cancer. patients and methods : we assessed eight ...
PURPOSE To compare the methylation status of tumor-associated genes by quantitative pyrosequencing and qualitative methylation-specific PCR (MSP) techniques and to correlate the results with clinicopathologic features and patients outcome to determine which method might have greater clinical utility. EXPERIMENTAL DESIGN The hypermethylation status of the retinoid acid receptor beta2 (RARbeta2...
The ability to determine nucleic acid sequences is one of the most important platforms for the detailed study of biological systems. Pyrosequencing technology is a relatively novel DNA sequencing technique with multifaceted unique characteristics, adjustable to different strategies, formats and instrumentations. The aims of this thesis were to improve the chemistry of the Pyrosequencing techniq...
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To explore the feasibility of pyrosequencing for quantitative differential gene expression analysis we have performed a comparative study of the results of the sequencing experiments to those obtained by a conventional DNA microarray platform. A conclusion from our analysis is that, over a threshold of 35 normalized reads per gene, the measurements of gene expression display a good correlation ...
Accurate serotyping of Streptococcus pneumoniae remains important to monitor the changes in seroepidemiology of the organism over time. Though several PCR-based systems have been developed for this purpose, the cross-reactivity within serogroups often limits discrimination between types. All serogroup 6 isolates can be identified using a multiplex PCR system; however, due to the high sequence h...
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