A novel protocol for production of multiple shoot tip clumps of Keelar nelli (Phyllanthus amarus Schum. & Thom.) were developed in vitro from shoot tip explants cultured on Murashige and Skoog (MS) medium containing only BAP or BAP with NAA, 3% sucrose and 0.8% agar. The addition of the following combination of growth substances 0.1mgLBAP and 0.05mgLNAA, caused an extensive proliferation of mul...

The genus Phyllanthus (Phyllanthaceae) is distributed in tropical and subtropical regions, and its members are widely used as medicinal plants in many countries. We analyzed the nucleotide sequences of the internal transcribed spacers of ribosomal DNA of 56 plant samples covering 23 Phyllanthus species collected from various habitats in Thailand. Based on the sequence alignment, we constructed ...

Phyllanthus amarus (PA) is a small herb indigenous to Amazon Basin .It is well known for its medicinal properties and widely used by oriental countries. It is reported to contain lignans,alkaloids,flavonoids,galloatnoids ,glycosides and alkaloids. It possesses antiviral, antiparasitic, antimalarial, antimicrobial, anticancerous, anti-diabetic and anti-Cholesterol agents. It acts on kidney stone...

Phyllanthin is a major bioactive lignan component of Phyllanthus amarus, with several known biological activities. This study dealt with the isolation and physicochemical characterization of phyllanthin. Phyllanthin was isolated from P. amarus leaves by column chromatography and purified by recrystallization to obtain phyllanthin crystals with a purity of more than 98%. UV, IR, MS, (1)H NMR and...

We examined contents of alimentary canal from pond-reared Rio Grande silvery minnows (Hybognathus amarus; n 5 183) to determine frequency of occurrence for foods consumed among small (9–20 mm), medium (21–35 mm), and large fish (36–75 mm). Among 183 fish, 19% (n 5 34) of alimentary canals were empty. Of those with food items (n 5 149), insects were the most common and were present in 66% of fis...

The herbal preparations of Phyllathus amarus has been used extensively in indigenous system of medicine for treating many infectious diseases. A great deal of interest has been generated on the protective effects of whole plant extracts of Phyllanthus against mutagens. The present study was designed to evaluate the modifying effect of Phyllanthus extract against X –ray induced chromosomal damag...