The usefulness of random amplified polymorphic DNA (RAPD) was examined as a potential tool to differentiate cryptic mosquito species. It proved to be a quick, effective means of finding genetic markers to separate two laboratory populations of morphologically indistinguishable African malaria vectors, Anopheles gambiae and An. arabiensis. In an initial screening of fifty-seven RAPD primers, 377...

This present work presents a robust system for the feature reduction, using Deoxyribonucleic Acid (DNA) primer. This system reaches up to 100% classes identification based on Support Vector Machines (SVM). In particular, the biochemical parameterization has 89 Random Amplified polymorphic DNA (RADP) primers of Pejibaye Palm races, and it has been reduced to 10 RADP primers. The development of t...

Turkey is one of the most important genetic resources of the date plum, Diospyros lotus, especially in the northeastern part of the country. Authenticating the identity of germplasm resources of D. lotus would be of great value for breeding. We examined the genetic variability of 11 D. lotus genotypes sampled from Coruh Valley in Turkey. One hundred and twenty-eight DNA markers were generated b...

Breast cancer is the most common cancer among women worldwide. Genetic alterations prevalent in breast cancer are still being elucidated. In this report, changes in 30 breast cancer tissues, in comparison with normal tissues from Thai patients, were analyzed by arbitrarily primed polymerase chain reaction (AP-PCR). Genetic instability was detected by DNA fingerprinting obtained with 13 of 60 ra...

The randomly amplified polymorphic DNA (RAPD) technique was used in the development of a fingerprinting (typing) and identification protocol for Legionella strains. Twenty decamer random oligonucleotide primers were screened for their discriminatory abilities. Two candidate primers were selected. By using a combination of these primers, RAPD analysis allowed for the differentiation between all ...

A study was conducted to investigate the relationship between the presence of localized bacterial infection and lameness in broiler fowl (Gallus gallus domesticus). Isolation of bacteria from the proximal femur, proximal tibia, and tibiotarsus from broilers with lameness revealed a probable association between lameness and the presence of Staphlylococcus aureus. Other potential pathogens, inclu...

Due to lack of morphological methods to identify sex at early stage in the plants with long juvenile period the application of molecular markers is expected to facilitate breeding program. The objective of this study is to identify molecular markers linked to sex determination of the plant Simarouba glauca which assists in crop improvement program. Random amplified polymorphic DNA primers were ...

Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of the five primers were statistically correlated (P < 0.001). The combined data from three primers wer...

Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiol...

Jinten hitam ( Nigella sativa ) is one of the medicinal plants in family Ranunculaceae, native to Mediterranean region and several regions Asia. The seed widely cultivated, distributed, used pharmaceuticals, cosmetics, food industries. Due similar morphology its potential adulterant such as Corchorus spp., N. seeds are susceptible adulteration substitution markets. Molecular markers have become...