Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

The glucocorticoid receptor (GR) is a well characterised sequence specific DNA binding protein. Functional mapping of rat GR has shown that it consists of a C-terminal ligand binding domain, a central DNA binding domain and an N-terminal transactivation domain (for a review, see 1). Comparison of the amino acid sequence of the N-terminal domains of the human, mouse and rat GR reveals a polyglut...

.....................................................................................................................6 Zusammenfassung ....................................................................................................8 Chapter 1: General Introduction................................................................................................11 Chapter 2: Inhibition of the m...

The incorporation of radioactively labeled dTTP or dATP by rat liver mitochondria in vitro is stimulated by factors present in the postmicrosomal fraction of rat and mouse tumors and in regenerating and fetal rat liver, but not in normal liver. The observed stimulation is completely abolished by ethidium bromide, a specific inhibitor of mitochondrial DNA polymerase in mammalian systems. The sti...

Tamoxifen (TAM) is used as the standard endocrine therapy for breast cancer patients and as a chemopreventive agent for women at high risk for this disease. Unfortunately, treatment of TAM increases the incidence of endometrial cancer; this may be due to the genotoxic damage induced by TAM metabolites. Formation of TAM-DNA adducts in rat liver correlates with the development of hepatocarcinoma....

The black rat (Rattus rattus) is an alien species that causes severe impact on island ecosystems, floras and faunas. main aim of this study was to determine the plant animal contributions diet in a pristine misty laurel forest area La Palma (Canary Islands). Our working hypothesis equally consumes plants animals (fully omnivorous animal) wherever it introduced, including habitats. A total 483 d...

Spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto ("WKY") rats are frequently employed in experimental studies of hypertension. Although both SHR and WKY rat strains have been presumed to be fully inbred, recent studies have revealed important biologic variability in WKY rats from different commercial sources. Genealogic evidence suggests that, in the United States, breeding s...