Single-molecule FRET is a powerful tool for probing the kinetic mechanism of a complex enzymatic reaction. However, not every reaction intermediate can be identified via a distinct FRET value, making it difficult to fully dissect a multistep reaction pathway. Here, we demonstrate a method using sequential kinetic experiments to differentiate each reaction intermediate by a distinct time sequenc...

Traditionally, reaction conversion and enantiomeric excess (% ee) of products are monitored using two separate HPLC or GC systems, one system for monitoring the reaction conversion and the other system for determining the % ee. In addition, in-process samples are usually sampled manually, limiting the continuous monitoring of reaction process. For the first time, a fully automated on-line achir...

The purpose of this study was to investigate the relationship between kinetic visual acuity and reaction time in college students. Eighteen male college students, mean age: 21.5±2.2 years, participated in this study. Static and kinetic visual acuity and simple and choice reaction time were measured. In addition, visual evoked potential using pattern reversal stimulus was recorded. These items w...

Mathematical modeling and simulation of biochemical reaction networks facilitates our understanding of metabolic and signaling processes. For closed, well-mixed reaction systems, it is straightforward to derive kinetic equations that govern the concentrations of the reactants and products. The usual way of deriving kinetic equations involves application of the principle of conservation of mass ...

reaction constants and heat release during thermophilic aerobic digestion of typical secondary and mixed sludges were determined. maximum reaction constant, determined by measuring the amounts of biodegradable volatile suspended solids (bvss) removed, occurred in the temperature range of 55-60 ºc. measurement of heat releases were carried out, at the same range, by the aid of a temperature prog...

the kinetic of the heterogeneous chemical reaction of aluminum hydroxide and fluosilicic acid was studied. it was found that the diffusion of the reactants through the porous silica coating to the aluminum hydroxide surface and the interfacial chemical reaction between the diffusing reactant and aluminum hydroxide platelets control the overall reaction rate. these two phenomena were studied and...

A new kinetic method for the determination of Hg(II) based on its inhibitory effect on the Brilliant green-sulfite reaction at pH 6.5 is proposed. The reaction is followed spectrophotometrically at 615 nm by measuring the change in absorbance at the fixed time of 3.5 min after the initiation of the reaction. Both the influence of the reaction variables and the interference of many ions have...