By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RTPCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transien...

background and objectives: kerman province, especially city of bam in the southeast part of iran, is epidemics for dry type cutaneous leishmaniasis (dtcl). this study was conducted to compare the effect of different treatments on parasite dna load following therapies using real-time pcr method. materials and methods: fifteen patients were divided into three groups under therapy with intralesio...

objectives: human cytomegalovirus (cmv) is a major life-threatening pathogen for hematopoietic stem cell transplant recipients. specific tests are used for the diagnosis and monitoring of cmv infection in transplant patients. this study evaluates the performance of pp65 antigenemia and qualitative pcr assays for monitoring cmv in such patients. methods: we analyzed 179 clinical samples from 41...

many of the video processing algorithms cannot be implemented in real time on general computers, due to their computational complexity. for an efficient implementation, a custom hardware that can be reconfigured for the algorithm, is used. in this paper a new acceleration hardware based on fpga elements is proposed. this hardware can be adapted with the processing algorithm through fpga design ...

background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...

background : enteroaggregative e. coli (eaec) is increasingly recognized as a cause of often persistent diarrhea in children and adults in both developing and developed countries. the aim of the present study was to investigate the presence and the frequency of eaec as etiologic agent of diarrhea in shiraz. methods : a total of 715 stool samples were collected from patients with diarrhea in shi...