نتایج جستجو برای: recombinant bacmid

تعداد نتایج: 111029  

Journal: :The Journal of general virology 2005
Dong Wu Fei Deng Xiulian Sun Hualin Wang Li Yuan Just M Vlak Zhihong Hu

The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene rep...

2017
Takashi Dojima Takuya Nishina Tatsuya Kato Tsuyoshi Uno Koichi Kato Hiroshi Ueda Enoch Y. Park

Human 29IJ6 IgG was expressed in silkworm using a Bombyx mori 25 nucleopolyhedrovirus (BmNPV) bacmid system. The mean amounts of 296IJ6 IgG 26 produced in larval hemolymph and whole pupae were 30.1 μg/larva and 78.0 μg/pupa,

Journal: :Virus research 2008
Oihane Simón Trevor Williams Primitivo Caballero Robert D Possee

Homologs to Autographa californica multiple nucleopolyhedrovirus (AcMNPV) open reading frame (ORF) 136 or Acp26 are present within almost all nucleopolyhedroviruses (NPVs). Two copies of the gene are found in some members of group II NPVs, suggesting that it may play an important role in transmission or replication. Phylogenetic analysis revealed that the predicted protein has some similarity w...

Journal: :Journal of virology 2010
Wenbi Wu A Lorena Passarelli

The Autographa californica multiple nucleopolyhedrovirus orf92 (p33), ac92, is one of 31 genes carried in all sequenced baculovirus genomes, thus suggesting an essential function. Ac92 has homology to the family of flavin adenine dinucleotide-linked sulfhydryl oxidases and is related to the ERV/ALR family of sulfhydryl oxidases. The role of ac92 during virus replication is unknown. Ac92 was ass...

Journal: :Protein engineering 2003
Stephen A Kaba Johannes C Hemmes Jan W M van Lent Just M Vlak Vishvanath Nene Anthony J Musoke Monique M van Oers

Theileria parva is an intracellular protozoan parasite that causes East Coast fever, a severe lymphoproliferative disease in cattle. Previous attempts to produce recombinant sporozoite surface antigen (p67) in bacterial or insect cells for vaccine purposes have not resulted in a correctly folded protein. Here, we report the expression of N- and C-terminal domains of p67 fused to the baculovirus...

Journal: :Protein expression and purification 2009
B Shay Y Gruenbaum-Cohen A S Tucker A L Taylor E Rosenfeld A Haze L Dafni Y Leiser E Fermon T Danieli A Blumenfeld D Deutsch

Tuftelin is an acidic protein expressed at very early stages of mouse odontogenesis. It was suggested to play a role during epithelial-mesenchymal interactions, and later, when enamel formation commences, to be involved in enamel mineralization. Tuftelin was also detected in several normal soft tissues of different origins and some of their corresponding cancerous tissues. Tuftelin is expressed...

Journal: :Bioscience, biotechnology, and biochemistry 2007
Yan-Hong Si Ming-Gang Fang Yi Huang Fang-Liang Zheng Ting Li Zhi-Hong Hu Han-Zhong Wang

Genetically modified baculoviruses offer a promising alternative to chemical insecticides in the control of agricultural and forest insect pests. A novel bacmid, HaBacYH6, was constructed in which the ecdysteroid UDP-glucosyltransferase gene (egt) was replaced with a bacterial replication cassette containing a mini-F replicon, a kanamycin resistance gene, and the attTn7 site. Insertion of the e...

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