Translation of total mRNA in heterologous protein-synthesizing systems is often employed as an indirect means of assessing relative mRNA concentrations. However, it is well known that the efficiency of translation of specific mRNAs differs. One such example is the poor translational efficiency of conalbumin mRNA relative to ovalbumin mRNA. In this report we have studied the translation of conal...

Recent advances in meta-omics and particularly metatranscriptomic approaches have enabled detailed studies of the structure and function of microbial communities in many ecosystems. Molecular analyses of peat soils, ecosystems important to the global carbon balance, are still challenging due to the presence of coextracted substances that inhibit enzymes used in downstream applications. We sampl...

objective: orexin is a hypothalamic orexigenic neuropeptide, which third cerebral injection of it mainly exerts inhibitory effects on reproductive functions. it increases significantly the aromatase (cyp19) gene expression in the hypothalamus of male rats. aromatase is an enzyme which converts androgens to estradiol in the hypothalamus of rats. prenatal or neonatal exposure of females to testos...

This paper addresses the expression of the epidermal growth factor (EGF) gene by human breast tumor biopsy samples. Northern analysis was used to demonstrate the presence of an approximately 5-kilobase mRNA which specifically hybridized with radiolabeled human EGF complementary DNA in some human breast tumor biopsy samples. Quantitation of EGF mRNA in 60 human breast tumor biopsies using the RN...

Abstract The precise regulatory role in protein synthesis by facilitating interactions with mRNA codons for various tRNA modifications is unclear. We previously reported that enhanced green fluorescent (GFP) reduced GFP expression human methionine-conjugated initiator (tRNAi)/tRNA synthetase pairs under methionine-deficient conditions. Here, we investigated the effect of non-formylated Escheric...

PURPOSE The purpose of this study is to attempt to characterize patients with unfavorable clinical outcome by the relative mRNA levels of ABC transporter expression in their tumor samples and to examine whether relative mRNA levels of each of the ABC transporters can be a useful predictor of progression-free survival in advanced ovarian carcinoma. EXPERIMENTAL DESIGN We examined tumor samples...

in order to estimate responses of growth rate and growth hormone of clarias gariepinus to stocking density, fish (initial weight 30.71 ± 0.89 g) were stocked in 120-l tanks at densities of 35, 65, 95, and 125 kg/m3 for 60 days. on day 30 and 60, the fish growth rates were measured, pituitary growth hormone (gh) mrna levels and serum gh concentrations were examined by real-time qpcr and elisa te...

 In this paper by use of mathematical modeling of an observer [14,15] the notion of relative information functional for relative dynamical systemson compact metric spaces is presented. We extract the information function ofan ergodic dynamical system (X,T) from the relative information of T fromthe view point of observer χX, where X denotes the base space of the system.We also generalize the in...

PURPOSE To evaluate the mRNA and protein expressions of NLRP3 inflammasome and its downstream inflammatory factors in human dry eye. METHODS We recruited 54 patients with Sjögren's syndrome dry eye (SSDE), 50 patients with non-Sjögren's syndrome dry eye (NSSDE), and 46 healthy controls. Tear film breakup time (TBUT), Schirmer I test, and fluorescein staining (FL) were performed on all subject...

BACKGROUND Cholinergic signaling via muscarinic acetylcholine receptors (mAChR) is known to influence various physiological functions. In bone, M3 mAChR and M5 mAChR were identified on the membrane of osteoblast-like cells. M3 mAChR seems to be particularly relevant for bone physiology, as signaling via this receptor was reported to increase bone formation and decrease bone resorption. Thus, in...