A series of point mutants were generated in the human c-fos dyad symmetry element (DSE), found within the c-fos serum response element, to study the sequence requirements for its interaction with the human HeLa cell serum response factor (SRF). Plasmids that contain base substitutions within a core CC(A/T)6GG motif in the center of the DSE did not compete, or competed very poorly, with the wild...

background and objectives: psychrotrophic bacteria can produce enzymes at low temperatures; this provides a wide biotechnological potential, and offers numerous economical advantages over the use of mesophilic bacteria. in this study, extracellular protease production by psychrotrophic rheinheimera sp. (km459533) was optimized by the response surface methodology. materials and methods: the cult...