نتایج جستجو برای: restriction site mapping

تعداد نتایج: 609052  

Journal: :Journal of Computer and System Sciences 2002

Journal: :Nucleic acids research 1990
M W Germann B W Kalisch P Lundberg H J Vogel J H van de Sande

We have investigated loop-induced structural perturbation of the stem structure in hairpins d(GAATTCXnGAATTC) (X = A, T and n = 3, 4, 5 and 6) that contain an EcoRI restriction site in close proximity to the hairpin loop. Oligonucleotides containing either a T3 or a A3 loop were not hydrolyzed by the restriction enzyme and also showed only weak binding to EcoRI in the absence of the cofactor Mg...

Journal: :Journal of medical genetics 1987
A V Hill S L Thein B Mavo D J Weatherall J B Clegg

Analysis of DNA from members of a Melanesian family from Papua New Guinea with haemoglobin (Hb) H disease revealed that all four alpha globin genes are intact in affected subjects. Study of restriction enzyme site and length polymorphisms and the use of oligonucleotide probes indicated that the molecular basis of this Papuan form of non-deletion Hb H disease differs from the previously describe...

1998
Andrew Keller

Restriction mapping involves cleaving DNA clones at specific sites with enzymes to produce a set of fragments. These fragments have characteristic lengths which together constitute a multiset (a set allowing repeated elements) called the “fingerprint” of the clone. Clones that overlap with each other share common restriction fragments as long as the region of overlap contains at least two enzym...

Journal: :Journal of clinical microbiology 1998
R K Aakre A Jenkins B E Kristiansen L O Froholm

Forty-two Neisseria meningitidis isolates were obtained from patients with meningococcal disease in the Norwegian county of Telemark (January 1987 to March 1995), and all were compared by PCR amplicon restriction endonuclease analysis (PCR-AREA) of the dhps gene, chromosomal DNA fingerprinting, and serological analysis. PCR-AREA divided the isolates into 11 classes, of which 4, comprising 15, 8...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1998
P Lauer S S Schneider A Gnirke

RecA-assisted restriction endonuclease (RARE) cleavage is an "Achilles' heel" approach to restriction mapping whereby a RecA-protein-oligodeoxynucleotide complex protects an individual restriction site from methylation, thus limiting subsequent digestion to a single, predetermined site. We have used RARE cleavage to cut yeast artificial chromosomes (YACs) at specific EcoRI sites located within ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Robert Riehn Manchun Lu Yan-Mei Wang Shuang Fang Lim Edward C Cox Robert H Austin

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites wit...

Journal: :Journal of clinical microbiology 2006
Therdsak Prammananan Saranya Phunpruch Nipa Tingtoy Somboon Srimuang Angkana Chaiprasert

A total of 227 clinical Mycobacterium avium complex isolates from Thailand were differentiated into species and types by using PCR-restriction enzyme analysis of hsp65. The distribution of types showed the predominance of M. avium I (77%) in blood specimens, whereas M. intracellulare I was more commonly found in pulmonary specimens (44.2%). In addition, infections with M. avium were more likely...

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