Nuclear export of unspliced and incompletely spliced human immunodeficiency virus type 1 mRNA is mediated by the viral Rev protein. Rev binds to a structured RNA motif known as the Rev-response element (RRE), which is present in all Rev-dependent transcripts, and thereby promotes entry of the ribonucleoprotein complex into the nuclear-export pathway. Recent evidence indicates that a dimerizatio...

101 Recording of Absences (Attendance) [2] [rev. Oct 1999] 102 Hours of Work [3] [rev. Oct 1999] 103 Vacation Leave [4] [rev. Aug 2004] 104 Sick Leave [5] [rev. Nov 1999] 105 Membership Fees [6] [rev. Oct 1999] 106 Termination [7] [rev. Oct 1999] 107 Acting Pay [8] [rev. Nov 1999] 108 Flexible Spending Credits [9] [rev. 2017] 109 Self-Funded Leave, see Policy 501Leave of Absence [10] [rev. Feb ...

101 Recording of Absences (Attendance) [1] [rev. Oct 1999] 102 Hours of Work [2] [rev. Oct 1999] 103 Vacation Leave [3] [rev. Aug 2004] 104 Sick Leave [4] [rev. Nov 1999] 105 Membership Fees [5] [rev. Oct 1999] 106 Termination [6] [rev. Oct 1999] 107 Acting Pay [7] [rev. Nov 1999] 108 Professional Development Reimbursement [8] [rev. Jul 2005] 109 Self-Funded Leave, see Policy 501Leave of Absenc...

Human immunodeficiency virus type I encodes a regulatory protein, termed Rev, which is associated with the appearance of unspliced and partially spliced viral RNAs in the cytoplasm. Rev is believed to function via interaction with a sequence element in the env region of the viral RNA, termed the Rev-responsive element (RRE). In this study, we use a stably transfected, Rev-producing mouse cell l...

HIV replication requires nuclear export of unspliced and singly spliced viral transcripts. Although a unique RNA structure has been proposed for the Rev-response element (RRE) responsible for viral mRNA export, how it recruits multiple HIV Rev proteins to form an export complex has been unclear. We show here that initial binding of Rev to the RRE triggers RNA tertiary structural changes, enabli...

Oligomerization of the HIV-1 protein Rev on the Rev Response Element (RRE) regulates nuclear export of genomic viral RNA and partially spliced viral mRNAs encoding for structural proteins. Single-molecule fluorescence spectroscopy has been used to dissect the multistep assembly pathway of this essential ribonucleoprotein, revealing dynamic intermediates and the mechanism of assembly. Assembly i...

The HIV-1 REV protein binds to the stem II region of the REV-responsive element (RNA). Studies to further define the RNA sequence and structure specifically bound by REV protein identify a minimal RNA element of 40 nucleotides. Analysis of RNA fragments by gel retardation and filter binding suggest that a core element composed of one particular stem with flanking sequences capable of forming a ...

The aim of this study was to prepare liposomes of acyclovir (ACY) by thin layer evaporation (TLE) and reverse phase evaporation (REV) methods. Twenty-seven batches of liposomes from each method were prepared using technique of three variables at three levels (33) factorial design. Drug/Lipid (molar ratio), hydration volume and hydration time were considered three independent variables in TLE me...

Rev, a major regulatory protein of human immunodeficiency virus type 1, has been demonstrated to shuttle between the nucleus and cytoplasm of infected cells. The fate of the Rev protein in living cells was evaluated by pulse-chase experiments using a transient Rev expression system. Sixteen hours after chasing with unlabelled amino acids, 45% of the labelled Rev was still present, which clearly...

Astrocytes are cellular targets for the human immunodeficiency virus (HIV) that limit virus production, owing, at least in part, to the diminished functionality of the viral post-transcriptional stimulatory factor Rev. To understand the trafficking process in astrocytes, we compared nucleocytoplasmic transport of Rev and various proteins with well-characterized nucleocytoplasmic transport featu...