background and aims: certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. reverse transcription-polymerase chain reaction (rt-pcr) has been documented as an alternative assay for certificat...

People's lives could be in danger if a contagious disease spreads quickly, Corona-2019 virus (COVID-19) is one. The coronavirus epidemic rapidly spread over the world. Corona has had major impact on health of populations and healthcare systems all RT-PCR (RT-Reverse transcription, PCR-polymerase chain reaction) testing can benefit from use computed tomography images. Most available methods larg...

  Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...

: The coronavirus disease 2019 (COVID-19) is a new infectious disease, firstly appeared in Wuhan city and has rapidly spread to 114 countries outside China, which receiving worldwide attention. As two important means of examination, computed tomography (CT) real-time reverse transcription polymerase chain reaction (RT-PCR) have always been controversial the clinical diagnosis COVID-19 pneumonia...

Rabies are one of the major zoonoses in Mali. The control this disease is based on use effective diagnostic techniques, management human cases after exposure and medical prophylaxis animals. Diagnosis usually made by detection viral antigen direct immunofluorescence (IF). Due to increasing variability genus Lyssavirus observed, it was found necessary a complementary technique, namely reverse tr...

Abstract A multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) assay was developed to enable the simultaneous detection and differentiation of four viruses that infect passion fruit, citrus-associated rhabdovirus (CiaRV), East Asian Passiflora virus (EAPV), latent (PLV), Telosma mosaic (TeMV). The optimized parameters included primer concentration, annealing temperature...

To describe the characteristics of neonates born to mothers with confirmed Covid-19 infection. A descriptive retrospective study using medical record data patients treated in neonatal intensive care unit (NICU) Dr. Soetomo Regional General Hospital from January 1 July 24, 2021. total 174 were positive COVID-19 reverse transcription-polymerase chain reaction (RT-PCR) swab test results. 11 RT-PCR...

west nile virus (wnv) is an important zoo-notic agent having a wide host range. due to its emergence with increased virulence in a wide geographical range, its monitoring becomes im-perative. development of more rapid and sensitive molecular techniques for instance reverse trans-criptase-polymerase chain reaction (rt-pcr), reverse transcription loop-mediated isothermal amplification (rt-lamp) a...

The COVID-19 pandemic has underscored the shortcomings in deployment of state-of-the-art diagnostics platforms. Although several polymerase chain reaction (PCR)-based techniques have been rapidly developed to meet growing testing needs, such often need samples collected through a swab, use RNA extraction kits, and expensive thermocyclers order successfully perform test. Isothermal amplification...