نتایج جستجو برای: reverse transcription polymerase chain reaction

تعداد نتایج: 957608  

Journal: :European journal of histochemistry : EJH 2003
M Boniotto O Radillo L Braida D Pirulli A Città T Not A Amoroso S Crovella

2016
Hong Zhang Haiqing Li Yan Liu Qingyan Li Yufang Bi Guiqing Fang

The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus (MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmid in vitro. Following tr...

2011
Wolfgang Poeppl Harald Herkner Heinz Burgmann Tom Pustelnik Gerhard Mooseder Theresia Popow-Kraupp Monika Redlberger-Fritz

To investigate the diagnostic accuracy of the QuickVue® Influenza A+B rapid test we conducted a prospective observational study in which this rapid test was compared with a real-time reverse transcription polymerase chain reaction (RT-PCR) for pandemic influenza A H1N1 (2009) infection in Austrian adults. The sensitivity, specificity, and positive and negative predictive values of the QuickVue ...

Journal: :Yi chuan = Hereditas 2007
Jia-Da Yang Hai-Tao Shang Hong Wei Wan-Shen Yang Xin Liu

CYP3A29 is the most important key enzyme for drug metabolism in pig's liver. Research of the characters of CYP3A29 mRNA expression in Chinese experimental miniature pig's livers is significant for evaluating it whether suitable for experimental model for drug metabolism mediated by human CYP3A4 enzyme. In this study, the levels of CYP3A29 mRNA expression in livers of Bama miniature pigs, Guizho...

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2011
Pauline M Lucas Oliver W Morgan Thomas F Gibbons Alicia C Guerrero Genny M Maupin Jenny L Butler Linda C Canas Vincent P Fonseca Sonja J Olsen Victor H MacIntosh

Clinicians frequently use influenza rapid antigen tests for diagnostic testing. We tested nasal wash samples from 1 April to 7 June 2009 from 1538 patients using the QuickVue Influenza A+B (Quidel) rapid influenza antigen test and compared the results with real-time reverse transcription polymerase chain reaction (rRT-PCR) assay (gold standard). The prevalence of 2009 pandemic influenza A (pH1N...

2016
Peter E. Kammerer Jennifer M. Radin Anthony W. Hawksworth Chris A. Myers Gary T. Brice

The Quidel Sofia Influenza A+B Fluorescent Immunoassay was used to test nasal swab specimens from patients with influenza-like illness at US-Mexico border-area clinics in the 2012-2013 and 2013-2014 influenza seasons. Compared with real-time reverse transcription polymerase chain reaction, the overall sensitivities and specificities were 83% and 81%, and 62% and 93%, respectively.

ژورنال: :مجله دانشکده پزشکی اصفهان 0
فرزاد کتیرائی استادیار، گروه پاتوبیولوژی، دانشکده ی دامپزشکی، دانشگاه تبریز، تبریز، ایران اسماعیل بابایی استادیار، گروه بیولوژی، دانشکده ی علوم طبیعی، دانشگاه تبریز، تبریز، ایران عادل قادری دامپزشک، گروه پاتوبیولوژی، دانشکده ی دامپزشکی، دانشگاه تبریز، تبریز، ایران جواد اشرفی هلان استاد، گروه پاتوبیولوژی، دانشکده ی دامپزشکی، دانشگاه تبریز، تبریز، ایران

چکیده مقدمه:   ژن های camca1 و hsp90 در ایجاد مرگ سلولی برنامه ریزی شده در سلول های قارچی نقش دارند. هدف از انجام این مطالعه، بررسی اثرات ضد قارچی نانوکورکومین دندروزومی علیه گونه های candida و بررسی اثر این دارو بر بیان این ژن ها بود. روش ها: حساسیت چهار گونه ی candida به کورکومین و نانوکورکومین طبق روش clsim27-s4 مورد بررسی قرار گرفت. در ادامه، بیان ژن های hsp90 و camca1 در سلول های تیمار شده...

Journal: :avicenna journal of medical biotechnology 0

west nile virus (wnv) is an important zoo-notic agent having a wide host range. due to its emergence with increased virulence in a wide geographical range, its monitoring becomes im-perative. development of more rapid and sensitive molecular techniques for instance reverse trans-criptase-polymerase chain reaction (rt-pcr), reverse transcription loop-mediated isothermal amplification (rt-lamp) a...

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